Detail Information for IndEnz0002019369
IED ID IndEnz0002019369
Enzyme Type ID protease019369
Protein Name Calpain-5
EC 3.4.22.-
Sex-determining transformer protein 3
Gene Name tra-3 clp-5 LLC1.1
Organism Caenorhabditis elegans
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans
Enzyme Sequence MTRSEKTRHFGNQNYEKLRKICIKKKQPFVDTLFPPTNQSLFLEQRQSSDIVWKRPGELHPDPHLFVEGASPNDVTQGILGNCWFVSACSALTHNFKLLAQVIPDADDQEWSTKHAYAGIFRFRFWRFGKWVEVVIDDLLPTRDGKLLFARSKTPNEFWSALLEKAFAKLYGCYENLVGGHLSDALQDVSGGVAETLHVRKFLKDDPNDTELKLFNDLKTAFDKGALVVAAIAARTKEEIEESLDCGLVKGHAYAVSAVCTIDVTNPNERSFTSFIMGSKRKQNLIRLQNPWGEKEWNGAWSDDSPEWQNVSASQLSTMGVQPANSDSDDGDFWMPWESFVHYFTDISLCQLFNTSVFSFSRSYDEQIVFSEWTTNGKKSGAPDDRAGGCHNFKATFCNNPQYIFDIPSPNCSVMFALIQNDPSEGLKKREPFVTIGMHVMKVENNRQYRVHTAMHPIAISDYASGRSVYLHLQSLPRGRYLLIPTTFAPKEQTLFMLRVYSDEHIHFSPLTKHAPKLGLLKCKSAQSVTRLTIHGVDFNSASTGTHNVYAILKDSRKSFRTKTLSGVKSIQWDEQFLFHKSKNRQQYKIEVWEDRKMARDHLLAQSVIIALIDNENRDTTLQLTDPRGTVIGTVSVTVSAFDDPMYL
Enzyme Length 648
Uniprot Accession Number Q22036
Absorption
Active Site ACT_SITE 83; /evidence=ECO:0000269|PubMed:10783162; ACT_SITE 252; /evidence=ECO:0000250|UniProtKB:Q07009; ACT_SITE 290; /evidence=ECO:0000250|UniProtKB:Q07009
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding
EC Number 3.4.22.-
Enzyme Function FUNCTION: Required for the correct female sexual development of the soma and germline in hermaphrodite animals, while being fully dispensable in males. Has calcium-dependent proteolytic activity and is involved in the cleavage of tra-2, for which it acts as a potentiator. Capable of calcium-dependent autolysis (PubMed:10783162). Part of the necrosis cell death pathway (PubMed:12410314). Required for necrosis of intestinal cells induced by B.thuringiensis endotoxin Cry6Aa (PubMed:26795495). {ECO:0000269|PubMed:10783162, ECO:0000269|PubMed:12410314, ECO:0000269|PubMed:26795495}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Domain (2); Mutagenesis (2); Region (1)
Keywords Autocatalytic cleavage;Calcium;Differentiation;Hydrolase;Necrosis;Protease;Reference proteome;Sexual differentiation;Thiol protease
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 10778742; 12097347; 12529643; 17164286; 17335351; 17889653; 19343510; 20439774; 21177967; 21367940; 22253611; 22267497; 22286215; 22347378; 22560298; 23800452; 24884423; 25487147; 560330; 6593563; 7713420;
Motif
Gene Encoded By
Mass 73,614
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda