| IED ID | IndEnz0002019369 |
| Enzyme Type ID | protease019369 |
| Protein Name |
Calpain-5 EC 3.4.22.- Sex-determining transformer protein 3 |
| Gene Name | tra-3 clp-5 LLC1.1 |
| Organism | Caenorhabditis elegans |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Nematoda (roundworms) Chromadorea Rhabditida Rhabditina Rhabditomorpha Rhabditoidea Rhabditidae Peloderinae Caenorhabditis Caenorhabditis elegans |
| Enzyme Sequence | MTRSEKTRHFGNQNYEKLRKICIKKKQPFVDTLFPPTNQSLFLEQRQSSDIVWKRPGELHPDPHLFVEGASPNDVTQGILGNCWFVSACSALTHNFKLLAQVIPDADDQEWSTKHAYAGIFRFRFWRFGKWVEVVIDDLLPTRDGKLLFARSKTPNEFWSALLEKAFAKLYGCYENLVGGHLSDALQDVSGGVAETLHVRKFLKDDPNDTELKLFNDLKTAFDKGALVVAAIAARTKEEIEESLDCGLVKGHAYAVSAVCTIDVTNPNERSFTSFIMGSKRKQNLIRLQNPWGEKEWNGAWSDDSPEWQNVSASQLSTMGVQPANSDSDDGDFWMPWESFVHYFTDISLCQLFNTSVFSFSRSYDEQIVFSEWTTNGKKSGAPDDRAGGCHNFKATFCNNPQYIFDIPSPNCSVMFALIQNDPSEGLKKREPFVTIGMHVMKVENNRQYRVHTAMHPIAISDYASGRSVYLHLQSLPRGRYLLIPTTFAPKEQTLFMLRVYSDEHIHFSPLTKHAPKLGLLKCKSAQSVTRLTIHGVDFNSASTGTHNVYAILKDSRKSFRTKTLSGVKSIQWDEQFLFHKSKNRQQYKIEVWEDRKMARDHLLAQSVIIALIDNENRDTTLQLTDPRGTVIGTVSVTVSAFDDPMYL |
| Enzyme Length | 648 |
| Uniprot Accession Number | Q22036 |
| Absorption | |
| Active Site | ACT_SITE 83; /evidence=ECO:0000269|PubMed:10783162; ACT_SITE 252; /evidence=ECO:0000250|UniProtKB:Q07009; ACT_SITE 290; /evidence=ECO:0000250|UniProtKB:Q07009 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.4.22.- |
| Enzyme Function | FUNCTION: Required for the correct female sexual development of the soma and germline in hermaphrodite animals, while being fully dispensable in males. Has calcium-dependent proteolytic activity and is involved in the cleavage of tra-2, for which it acts as a potentiator. Capable of calcium-dependent autolysis (PubMed:10783162). Part of the necrosis cell death pathway (PubMed:12410314). Required for necrosis of intestinal cells induced by B.thuringiensis endotoxin Cry6Aa (PubMed:26795495). {ECO:0000269|PubMed:10783162, ECO:0000269|PubMed:12410314, ECO:0000269|PubMed:26795495}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Domain (2); Mutagenesis (2); Region (1) |
| Keywords | Autocatalytic cleavage;Calcium;Differentiation;Hydrolase;Necrosis;Protease;Reference proteome;Sexual differentiation;Thiol protease |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 10778742; 12097347; 12529643; 17164286; 17335351; 17889653; 19343510; 20439774; 21177967; 21367940; 22253611; 22267497; 22286215; 22347378; 22560298; 23800452; 24884423; 25487147; 560330; 6593563; 7713420; |
| Motif | |
| Gene Encoded By | |
| Mass | 73,614 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |