| IED ID | IndEnz0003000005 |
| Enzyme Type ID | pectinase000005 |
| Protein Name |
Polygalacturonase ADPG2 AtADPG2 PG ADPG2 EC 3.2.1.15 Pectinase ADPG2 Protein ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 |
| Gene Name | ADPG2 PGAZAT At2g41850 T11A7.5 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MARCTNLVTVFLLWALLMFSWCKASRISPNVYDHSYKRFKSDSLIKRREDITGLRSFVRASLRTPTTVSVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGPCNSILTVQIFGTLSASQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIKGGKATCTNANVVDKGAVLPQCNST |
| Enzyme Length | 433 |
| Uniprot Accession Number | Q8RY29 |
| Absorption | |
| Active Site | ACT_SITE 264; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052; ACT_SITE 287; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
| DNA Binding | |
| EC Number | 3.2.1.15 |
| Enzyme Function | FUNCTION: Polygalacturonase involved in cell separation in the final stages of pod shatter, in anther dehiscence and in floral organ abscission. {ECO:0000269|PubMed:17928369, ECO:0000269|PubMed:19168715}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Erroneous gene model prediction (1); Repeat (5); Sequence conflict (8); Signal peptide (1) |
| Keywords | Cell wall;Cell wall biogenesis/degradation;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal |
| Interact With | |
| Induction | INDUCTION: Up-regulated by ethylene. {ECO:0000269|PubMed:11842157, ECO:0000269|PubMed:17928369}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..24; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12221970; 15047898; 15235117; 15272001; 15860015; 17337630; 17496113; 18192438; 20466844; 20736450; 22992509; 23056537; 23509178; 27379143; 27618493; 32278327; 32484317; |
| Motif | |
| Gene Encoded By | |
| Mass | 46,623 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |