| IED ID | IndEnz0003000007 |
| Enzyme Type ID | pectinase000007 |
| Protein Name |
Exopolygalacturonase ExoPG EC 3.2.1.67 Galacturan 1,4-alpha-galacturonidase Pectinase |
| Gene Name | PG1; PG2; PG3; PG6; PG14 |
| Organism | Zea mays (Maize) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae Liliopsida Petrosaviidae commelinids Poales Poaceae PACMAD clade Panicoideae Andropogonodae Andropogoneae Tripsacinae Zea Zea mays (Maize) |
| Enzyme Sequence | MACTNNAMRALFLLVLFCIVHGEKEESKGIDAKASGPGGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTIQVDGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNAKGSTKGCLKELACF |
| Enzyme Length | 410 |
| Uniprot Accession Number | P26216 |
| Absorption | |
| Active Site | ACT_SITE 233; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O74213; ACT_SITE 256; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=[(1->4)-alpha-D-galacturonosyl](n) + H2O = [(1->4)-alpha-D-galacturonosyl](n-1) + alpha-D-galacturonate; Xref=Rhea:RHEA:14117, Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14572, ChEBI:CHEBI:15377, ChEBI:CHEBI:58658, ChEBI:CHEBI:140523; EC=3.2.1.67; |
| DNA Binding | |
| EC Number | 3.2.1.67 |
| Enzyme Function | FUNCTION: May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (3); Glycosylation (4); Natural variant (1); Repeat (5); Signal peptide (1) |
| Keywords | Cell wall;Cell wall biogenesis/degradation;Direct protein sequencing;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. Secreted, cell wall. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..22; /evidence=ECO:0000269|PubMed:1932692 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,444 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:14117 |
| Cross Reference Brenda |