| IED ID | IndEnz0003000016 |
| Enzyme Type ID | pectinase000016 |
| Protein Name |
Endopolygalacturonase B EC 3.2.1.15 Pectinase B Polygalacturonase B |
| Gene Name | pgaB pecB AO090023000161 |
| Organism | Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus oryzae (Yellow koji mold) Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold) |
| Enzyme Sequence | MQLLQSSVIAATVGAALVAAVPVELKARDSCTFTSAADAKSGKTSCSTITLSNIEVPAGETLDLTGLNDGTTVIFSGETTFGYKEWEGPLISVSGTNIKVQQASGAKIDGDGSRWWDGKGGNGGKTKPKFFYAHKLDSSSITGLQIYNTPVQGFSIQSDNLNITDVTIDNSAGTAEGHNTDAFDVGSSTYINIDGATVYNQDDCLAINSGSHITFTNGYCDGGHGLSIGSVGGRSDNTVEDVTISNSKVVNSQNGVRIKTVYDATGTVSNVKFEDITLSGITKYGLIVEQDYENGSPTGTPTNGIKVSDITFDKVTGTVESDATDIYILCGSGSCTDWTWSGVSITGGKTSSKCENVSTGASC |
| Enzyme Length | 363 |
| Uniprot Accession Number | P35335 |
| Absorption | |
| Active Site | ACT_SITE 202; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052; ACT_SITE 224; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
| DNA Binding | |
| EC Number | 3.2.1.15 |
| Enzyme Function | FUNCTION: Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall. {ECO:0000269|PubMed:18562768}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (4); Glycosylation (2); Propeptide (1); Repeat (6); Sequence conflict (1); Signal peptide (1) |
| Keywords | Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,651 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |