| IED ID | IndEnz0003000018 |
| Enzyme Type ID | pectinase000018 |
| Protein Name |
Endopolygalacturonase B EC 3.2.1.15 P1/P3 PGL Pectinase B Polygalacturonase B |
| Gene Name | pgaB pecB P034_00381312 |
| Organism | Aspergillus flavus (strain ATCC MYA-384 / AF70) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Circumdati Aspergillus flavus Aspergillus flavus (strain ATCC MYA-384 / AF70) |
| Enzyme Sequence | MHFQLLGLAALGSLAAAAPAPSRTSELVERGSSCTFTSAAQASASAKSCSNIVLKNIAVPAGETLDLSKAKDGATITFEGTTTFGYKEWKGPLIRFGGNKITVTQAAGAVIDGQGSRWWDGKGTNGGKTKPKFIYAHKLQSSTIKGLHVKNSPVQVFSVQGNDVHLTDITIDNSDGDNNGGHNTDAFDVSESNGVYITGANVKNQDDCLAINSGENIEFTGATCSGGHGISIGSIGNRDSNTVKNVKVADSTVVDSDNGIRIKTISGATGSVSGVTYENITLKNIKKNGIVIEQDYKNGGPTGKPTTGVPITDLTVNGVTGSVASKATPVYILCGKGSCSDWTWKGVSISGGKKSDKCQNIPSGASC |
| Enzyme Length | 367 |
| Uniprot Accession Number | P41750 |
| Absorption | |
| Active Site | ACT_SITE 206; /note=Proton donor; ACT_SITE 228; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
| DNA Binding | |
| EC Number | 3.2.1.15 |
| Enzyme Function | FUNCTION: Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall. {ECO:0000269|PubMed:7574642}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (4); Glycosylation (1); Propeptide (1); Repeat (5); Sequence conflict (7); Signal peptide (1) |
| Keywords | Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Repeat;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | INDUCTION: Expressed in presence of pectin when glucose is absent. {ECO:0000269|PubMed:7574642}. |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,798 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |