| IED ID | IndEnz0003000025 |
| Enzyme Type ID | pectinase000025 |
| Protein Name |
Polygalacturonase PGA EC 3.2.1.15 Pectinase |
| Gene Name | pglA |
| Organism | Ralstonia solanacearum (Pseudomonas solanacearum) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Ralstonia Ralstonia solanacearum (Pseudomonas solanacearum) |
| Enzyme Sequence | MNHRYTLLALAAAALSAGAHATGTSVTAPWGEVAEPSLPADSAVCKTLSASITPIKGSVDSVDGNPANSQPDASRIQSAIDNCPAGQAVKLVKGSAGESGFLSGSLKLKSGVTLWIDTGVTLFASRNPADYDNGLGTCGTATTSNDKSCNALIVARDTAGSGIVGAGAIDGRGGSLVTSGPNANRLTWWDIAYLNKTKGLNQQNPRLIQTYNGSAFTLYGVTVQNSPNFHIVTTGTSGVTAWGIKIVTPSLAYAVAGYKCPSGSTPDKVTPATCFTPETVKNTDGFDPGQSTNVVLAYSYINTGDDHVAVKASSGPTRNLLFAHNHFYYGHGLSIGSETNTGVSNMLVTDLTMDGNDSSAGNGLRIKSDASRGGKVTNIVYDGICMRNVKEPLVFDPFYSSVKGSLYPNFTNIVVKNFHDLGSAKSIKRTMTFLGYKANKQKNPLTITLDNVVFDGTLPAFEGSHYGGPASPNGVHFTFGGTGPVSFADAIVTSSTTDVTVTGTPGTAAAVDCSKAFVPLKSVAPTSPI |
| Enzyme Length | 529 |
| Uniprot Accession Number | P20041 |
| Absorption | |
| Active Site | ACT_SITE 305; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052; ACT_SITE 331; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
| DNA Binding | |
| EC Number | 3.2.1.15 |
| Enzyme Function | FUNCTION: Contributes to the wilt disease production on tomato. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Region (1); Signal peptide (1) |
| Keywords | Cell wall biogenesis/degradation;Direct protein sequencing;Glycosidase;Hydrolase;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..21; /evidence=ECO:0000269|PubMed:2193922 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 54,636 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |