| IED ID | IndEnz0003000035 |
| Enzyme Type ID | pectinase000035 |
| Protein Name |
Polygalacturonase At1g48100 PG EC 3.2.1.15 Pectinase At1g48100 |
| Gene Name | At1g48100 F21D18.17 F21D18.18 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MRRLSGILVIYIISTVLFGHFTNVARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC |
| Enzyme Length | 475 |
| Uniprot Accession Number | Q949Z1 |
| Absorption | |
| Active Site | ACT_SITE 282; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052; ACT_SITE 305; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
| DNA Binding | |
| EC Number | 3.2.1.15 |
| Enzyme Function | FUNCTION: Polygalacturonase not involved in the final stages of pod shatter. {ECO:0000269|PubMed:19168715}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Compositional bias (1); Erroneous gene model prediction (1); Region (1); Repeat (5); Sequence conflict (1); Signal peptide (1) |
| Keywords | Cell wall;Cell wall biogenesis/degradation;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 17030626; 17478635; 17662035; 18650403; 18775970; 21245191; 23056537; 28974550; 29114014; 30394596; |
| Motif | |
| Gene Encoded By | |
| Mass | 51,353 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |