IED ID | IndEnz0003000035 |
Enzyme Type ID | pectinase000035 |
Protein Name |
Polygalacturonase At1g48100 PG EC 3.2.1.15 Pectinase At1g48100 |
Gene Name | At1g48100 F21D18.17 F21D18.18 |
Organism | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
Enzyme Sequence | MRRLSGILVIYIISTVLFGHFTNVARARYHYHKGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDSNYGC |
Enzyme Length | 475 |
Uniprot Accession Number | Q949Z1 |
Absorption | |
Active Site | ACT_SITE 282; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052; ACT_SITE 305; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
DNA Binding | |
EC Number | 3.2.1.15 |
Enzyme Function | FUNCTION: Polygalacturonase not involved in the final stages of pod shatter. {ECO:0000269|PubMed:19168715}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1); Compositional bias (1); Erroneous gene model prediction (1); Region (1); Repeat (5); Sequence conflict (1); Signal peptide (1) |
Keywords | Cell wall;Cell wall biogenesis/degradation;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 17030626; 17478635; 17662035; 18650403; 18775970; 21245191; 23056537; 28974550; 29114014; 30394596; |
Motif | |
Gene Encoded By | |
Mass | 51,353 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |