Detail Information for IndEnz0003000036
IED ID IndEnz0003000036
Enzyme Type ID pectinase000036
Protein Name Probable polygalacturonase At1g80170
PG
EC 3.2.1.15
Pectinase At1g80170
Gene Name At1g80170 F18B13.25
Organism Arabidopsis thaliana (Mouse-ear cress)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress)
Enzyme Sequence MSYSRGGTLVTLLLLLVVASSLALTANANSFESLLQLPRRQSRSTRPRSERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARLTLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYPPPCLSDDTSFLEQSVQSGITSAYL
Enzyme Length 444
Uniprot Accession Number Q94AJ5
Absorption
Active Site ACT_SITE 249; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052; ACT_SITE 272; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15;
DNA Binding
EC Number 3.2.1.15
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Erroneous gene model prediction (1); Repeat (6); Signal peptide (1)
Keywords Cell wall;Cell wall biogenesis/degradation;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal
Interact With Q8VZW1
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..28; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 16969662; 17526915; 18480120; 18723362; 18805951; 19582540; 20736450; 21798944; 23056537;
Motif
Gene Encoded By
Mass 48,172
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda