| IED ID | IndEnz0003000054 |
| Enzyme Type ID | pectinase000054 |
| Protein Name |
Probable endopolygalacturonase AFUA_1G17220 EC 3.2.1.15 Pectinase AFUA_1G17220 Polygalacturonase AFUA_1G17220 |
| Gene Name | AFUA_1G17220 |
| Organism | Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Neosartorya fumigata (Aspergillus fumigatus) Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus) |
| Enzyme Sequence | MLKLMGSLVLLASAAEVIASPAAEPVAPSTTLEKRAPCTFSGSNGAAAAMASQKACSTIVLSNVAVPAGTTLDLSDLADGTTVTFEGETTWGYQEWSGPLLKISGKNIKVKGASGATLNPDGARWWDGQGGNGGKTKPKFFAAHDLTSSSSITDLHILNTPVQAVSINGCDGLTITDITIDNSAGDTQGGHNTDAFDIGSSSNIIISGAKVYNQDDCVAVNSGTDITFTGGLCSGGHGLSIGSVGGRSDNTVENVSFTNSQVTNSDNGLRIKATKGKTGTIKGVTYSGITLSSIRKYGILIEQNYDGGDLKGDPTSGIPITDLTMQNISGKGAVASSGYNIAIVCGSGACSNWTWKSVEVTGGKTYGSCKNVPSVAQC |
| Enzyme Length | 378 |
| Uniprot Accession Number | Q4WR80 |
| Absorption | |
| Active Site | ACT_SITE 215; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052; ACT_SITE 237; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
| DNA Binding | |
| EC Number | 3.2.1.15 |
| Enzyme Function | FUNCTION: Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (4); Glycosylation (3); Propeptide (1); Repeat (5); Signal peptide (1) |
| Keywords | Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 38,425 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |