| IED ID | IndEnz0003000058 |
| Enzyme Type ID | pectinase000058 |
| Protein Name |
Polygalacturonase PG EC 3.2.1.15 Pectinase |
| Gene Name | G9 |
| Organism | Gossypium hirsutum (Upland cotton) (Gossypium mexicanum) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Malvales Malvaceae Malvoideae Gossypium Gossypium hirsutum (Upland cotton) (Gossypium mexicanum) |
| Enzyme Sequence | MAPHLNIVPSMFVLLLLFISASKVQSDAFDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIKHNGAEPATSQCLNVKPITSGKLNPIPCSGPVPKTPSATA |
| Enzyme Length | 407 |
| Uniprot Accession Number | Q39786 |
| Absorption | |
| Active Site | ACT_SITE 221; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O74213; ACT_SITE 244; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
| DNA Binding | |
| EC Number | 3.2.1.15 |
| Enzyme Function | FUNCTION: May function in the depolymerization of the pectin in its walls during pollen tube elongation, or in that of the pistil during pollination. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (3); Glycosylation (5); Repeat (5); Signal peptide (1) |
| Keywords | Cell wall;Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Secreted, cell wall {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..26; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,921 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |