| IED ID | IndEnz0003000061 |
| Enzyme Type ID | pectinase000061 |
| Protein Name |
Probable endopolygalacturonase NFIA_008150 EC 3.2.1.15 Pectinase NFIA_008150 Polygalacturonase NFIA_008150 |
| Gene Name | NFIA_008150 |
| Organism | Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus subgen. Fumigati Aspergillus fischeri Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus) |
| Enzyme Sequence | MLKLIGSLVLLASAAEVIASPLAESVAPSITLEKRASCTFSGSNGAAAAMASQKACSTIVLSNVAVPAGTTLDLSDLADGTTVIFEGETTWGYKEWSGPLLQISGKNIKVEGASGATLNPDGARWWDGQGGNGGKTKPKFFAAHGLTSSSSITNLHILNTPVQAVSINGCDGLTVTDMTIDDSAGDTQGGHNTDAFDIGSSSNIIISGAKVYNQDDCVAVNSGTGITFTGGLCSGGHGLSIGSVGGRSDNTVENVSFTNSQVTKSDNGLRIKASKGKTGTIKGITYSGITLSSIRKYGILIEQNYDGGDLKGDPTSGIPITDLTMENISGKGAVASSGHNIAIVCGSGACSNWTWKNVEVTGGQTYGSCENVPSVAQC |
| Enzyme Length | 378 |
| Uniprot Accession Number | A1D145 |
| Absorption | |
| Active Site | ACT_SITE 215; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052; ACT_SITE 237; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
| DNA Binding | |
| EC Number | 3.2.1.15 |
| Enzyme Function | FUNCTION: Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (4); Glycosylation (2); Propeptide (1); Repeat (5); Signal peptide (1) |
| Keywords | Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 38,359 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |