Detail Information for IndEnz0003000064
IED ID IndEnz0003000064
Enzyme Type ID pectinase000064
Protein Name Polygalacturonase
PG
EC 3.2.1.15
Pectinase
Gene Name PEPG1
Organism Penicillium expansum (Blue mold rot fungus)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Penicillium Penicillium expansum (Blue mold rot fungus)
Enzyme Sequence MILTRSVVLGFLGSASLALASPVAELAEGSRLTPRGSACSYSGTSGAAAAIAGKAGCSSITLNNVVVPAGTTLDLTGLASGTKVIFEGTTTFGYKQWAGPLISISGTNIQVSGASGHLIDGQGSRWWDGEGSNSKTNIKPKFFFAHSLKGSSTITGLNIKDSPVQVFSISGSSGLTISGVTIDNKNGDTNSLGHNTDGFDIGDSDSITITGATVYNQDDCLAINSGTNIVFSGGYCSGGHGLSIGSVGGRSNNVVETVHISSTQVVNSQNGVRVKAVSGATGTIKGVTFQDITLSGITSQGITIRQEYTNSGYTGSPTTGVPITGLTLNNVHGTVTSKGTDITIECGSSASCSGWTWTKVAVSGGKADVCKNAPSGTC
Enzyme Length 378
Uniprot Accession Number O59925
Absorption
Active Site ACT_SITE 218; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O74213; ACT_SITE 240; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15;
DNA Binding
EC Number 3.2.1.15
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Chain (1); Disulfide bond (4); Repeat (5); Signal peptide (1)
Keywords Cell wall biogenesis/degradation;Disulfide bond;Glycosidase;Hydrolase;Repeat;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..20; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 38,028
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.2.1.15;