| IED ID | IndEnz0003000076 |
| Enzyme Type ID | pectinase000076 |
| Protein Name |
Probable endopolygalacturonase II EPG-II EC 3.2.1.15 Pectinase 2 Polygalacturonase II PG-II Polygalacturonase X2 |
| Gene Name | pgaII pg2 pgaB AKAW_08560 |
| Organism | Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Aspergillus Aspergillus kawachii (White koji mold) (Aspergillus awamori var. kawachi) Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi) |
| Enzyme Sequence | MHSFASLLAYGLAAGATLASASPIEARDSCTFTTAAAAKAGKAKCSTITLDSIKVPAGTTLDLTGLTSGTKVIFEGTTTFDYEEWAGPLISMSGKDITVTGASGHLINCDGSRWWDGKGTSGKKKPKFFYAHGLDSSSITGLNIKNTPLMAFSVESDDITLTDITINNADGDSLGGHNTDAFDVGNSVGVNIIKPWVHNQDDCLAINSGENIWFTGGTCIGGHGLSIGSVGDRSNNVVKNVTIEHSTVSNSENAVRIKTISGATGSVSEITYSNIVMSGISDYGVVIQQDYEDGKPTGKPTNGVTITDVKLESVTGTVDSKATDIYLLCGSGSCSDWTWDDVKVTGGKKSSACKNYPSVASC |
| Enzyme Length | 362 |
| Uniprot Accession Number | Q8NK98 |
| Absorption | |
| Active Site | ACT_SITE 201; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052; ACT_SITE 223; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
| DNA Binding | |
| EC Number | 3.2.1.15 |
| Enzyme Function | FUNCTION: Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (4); Glycosylation (1); Propeptide (1); Repeat (5); Sequence conflict (1); Signal peptide (1) |
| Keywords | Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Reference proteome;Repeat;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..20; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,604 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |