IED ID | IndEnz0003000078 |
Enzyme Type ID | pectinase000078 |
Protein Name |
Endopolygalacturonase 1 EC 3.2.1.15 Clpg1 Pectinase |
Gene Name | PG1 |
Organism | Colletotrichum lindemuthianum (Bean anthracnose fungus) (Glomerella lindemuthiana) |
Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Glomerellales Glomerellaceae Colletotrichum Colletotrichum orbiculare species complex Colletotrichum lindemuthianum (Bean anthracnose fungus) (Glomerella lindemuthiana) |
Enzyme Sequence | MVSYLFVLGALASVAIASPVPELKARASCTFTDAASAIKGKASCTTIVLNNIAVPAGTTLDMTGLKSGTHVSFSGKTTFGYKEWEGPLISFSGSNVVIDGASGHSIDCQGSRWWDSKGGNGGKTKPKFFYAHSLKDSTIRGLHTLNTPVQAFSINGAANLGVYDVSVDNSAGDSAGGHNTDAFDVGSSTGVYISGADVKNQDDCLAVNSGTNITFTGGTCSGGHGLSIGSVGGRKDNVVKSVSITNSKIINSDNGVRIKTVAGATGPVSDITYSGITLSNIAKYGIVIEQDYENGSPTGKPTSGVPISGLTLSKISGSVSSSATPVYILCASCTNWKWSGVSVTGGKKSSKCTGIPSGSGAAC |
Enzyme Length | 363 |
Uniprot Accession Number | Q00446 |
Absorption | |
Active Site | ACT_SITE 202; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O74213; ACT_SITE 224; /evidence=ECO:0000255|PROSITE-ProRule:PRU10052 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
DNA Binding | |
EC Number | 3.2.1.15 |
Enzyme Function | FUNCTION: Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1); Disulfide bond (4); Glycosylation (1); Propeptide (1); Repeat (4); Signal peptide (1) |
Keywords | Cell wall biogenesis/degradation;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Repeat;Secreted;Signal;Zymogen |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..17; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 36,712 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |