| IED ID | IndEnz0003000084 |
| Enzyme Type ID | pectinase000084 |
| Protein Name |
Polygalacturonase PG EC 3.2.1.15 Pectinase |
| Gene Name | |
| Organism | Phaedon cochleariae (Mustard beetle) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Coleoptera Polyphaga Cucujiformia Chrysomeloidea Chrysomelidae (leaf beetles) Chrysomelinae Chrysomelini Phaedon Phaedon cochleariae (Mustard beetle) |
| Enzyme Sequence | MSIRLIAVLSAASIAVTSATPVADSSCTISSFDQVASVLAECTDIVVSNLEVPAGETLNLETKKKGVTITFEGKTTFAYKEWTGPLLRVKGKAITVVGAKGSVLDGQGQLYWDGKGGNGGIKKPKFFKIKATEGSHFKNINLLNCPVQVHSIDHSGPLTLSGWNIDVSQGDKDALGHNTDGFDINTTDQLTIEDTVVKNQDDCIAVNQGTNFLFNNLDCSGGHGLSLSVGTSHEIIKNTVRNVTFSNSVVRKSRNGIHIKTTYQFRGRYPWRMLTYSNIAMEGIWKYAVNVEQDYKKGKPTGIPVGNIPIKGLHLEKVTGTLTGEESTPVYIICADGACSNFNWSGVSFEGASHASNCSYVPTGYSC |
| Enzyme Length | 367 |
| Uniprot Accession Number | O97400 |
| Absorption | |
| Active Site | ACT_SITE 201; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O74213; ACT_SITE 223; /evidence=ECO:0000250|UniProtKB:O74213 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m).; EC=3.2.1.15; |
| DNA Binding | |
| EC Number | 3.2.1.15 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Disulfide bond (4); Glycosylation (4); Propeptide (1); Repeat (2); Signal peptide (1) |
| Keywords | Cell wall;Cell wall biogenesis/degradation;Cleavage on pair of basic residues;Digestion;Disulfide bond;Glycoprotein;Glycosidase;Hydrolase;Repeat;Secreted;Signal;Zymogen |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Secreted, cell wall {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 39,426 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |