| IED ID | IndEnz0003000098 |
| Enzyme Type ID | pectinase000098 |
| Protein Name |
Argininosuccinate synthase EC 6.3.4.5 Citrulline--aspartate ligase |
| Gene Name | argG Dda3937_00977 |
| Organism | Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Enterobacterales Pectobacteriaceae Dickeya Dickeya dadantii Dickeya dadantii (strain 3937) (Erwinia chrysanthemi (strain 3937)) |
| Enzyme Sequence | MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTANLGQPDEDDYDAIPRRAKEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGMTYFNTTPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELKIYKPWLDTDFIDELGGRQEMSEFMTTSGFDYKMSAEKAYSTDSNMLGATHEAKDLEFLNSSVKIVNPIMGVKFWDENVRIPAEEVTVRFERGHPVALNGQTFSDDVELLLEANRIGGRHGLGMSDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYHAHGRQLGRLLYQGRWFDPQALMLRDALQRWVASEITGEVTLELRRGNDYSILNTVSDNLTYKPERLTMEKGESVFSPDDRIGQLTMRNLDITDTREKLFNYVESGLIFSGNAGLPQVANPSLQDKSAK |
| Enzyme Length | 449 |
| Uniprot Accession Number | P0C1A1 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | BINDING 43; /note=ATP; via amide nitrogen and carbonyl oxygen; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 99; /note=Citrulline; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 129; /note=ATP; via amide nitrogen; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 131; /note=Aspartate; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 131; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 135; /note=Aspartate; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 135; /note=Citrulline; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 136; /note=Aspartate; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 136; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 139; /note=Citrulline; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 192; /note=Citrulline; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 194; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 201; /note=Citrulline; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 203; /note=Citrulline; /evidence=ECO:0000255|HAMAP-Rule:MF_00581; BINDING 280; /note=Citrulline; /evidence=ECO:0000255|HAMAP-Rule:MF_00581 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP + L-aspartate + L-citrulline = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+); Xref=Rhea:RHEA:10932, ChEBI:CHEBI:15378, ChEBI:CHEBI:29991, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57472, ChEBI:CHEBI:57743, ChEBI:CHEBI:456215; EC=6.3.4.5; Evidence={ECO:0000255|HAMAP-Rule:MF_00581}; |
| DNA Binding | |
| EC Number | 6.3.4.5 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. {ECO:0000255|HAMAP-Rule:MF_00581}. |
| nucleotide Binding | NP_BIND 17..25; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00581 |
| Features | Binding site (15); Chain (1); Nucleotide binding (1) |
| Keywords | ATP-binding;Amino-acid biosynthesis;Arginine biosynthesis;Cytoplasm;Ligase;Nucleotide-binding;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00581}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 50,176 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:10932 |
| Cross Reference Brenda |