| IED ID | IndEnz0004000023 |
| Enzyme Type ID | xylanase000023 |
| Protein Name |
Effector Vd424Y EC 3.2.1.8 Endo-1,4-beta-xylanase |
| Gene Name | VDAG_05042 |
| Organism | Verticillium dahliae (strain VdLs.17 / ATCC MYA-4575 / FGSC 10137) (Verticillium wilt) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Glomerellales Plectosphaerellaceae Verticillium Verticillium dahliae (Verticillium wilt) Verticillium dahliae (strain VdLs.17 / ATCC MYA-4575 / FGSC 10137) (Verticillium wilt) |
| Enzyme Sequence | MVSFTSLLAAFSVVSGVLTSPIAVVPEVNTALAKRTPSSTGTSGGFYYSFWTDTPNSVTYTNGDAGKFSVSWKNNNGNHVGGKGWRTGSARTIKYSGSYKPNGNSYLAIYGWTRSPLIEYYIVESFGTYNPSTGATSKGQFTVDGSVYDLYTSTRTNAPSIEGTRTFTQFWSVRRTKRTSGSVNTGAHFAAWKKAGMNLGSHDYQILAVEGYKSSGSATMTVS |
| Enzyme Length | 223 |
| Uniprot Accession Number | G2X4G0 |
| Absorption | |
| Active Site | ACT_SITE 119; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01097; ACT_SITE 210; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01097 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000255|PROSITE-ProRule:PRU01097}; |
| DNA Binding | |
| EC Number | 3.2.1.8 |
| Enzyme Function | FUNCTION: Secreted effector that localizes to the host nucleus to contribute to the virulence process (PubMed:34233072). Induces host innate immunity responses; triggers BAK1-and SOBIR1-dependent cell death, salicylic acid signaling and jasmonic acid signaling (PubMed:34233072). {ECO:0000269|PubMed:34233072}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. {ECO:0000255|PROSITE-ProRule:PRU01097}. |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Mutagenesis (2); Region (1); Signal peptide (1) |
| Keywords | Carbohydrate metabolism;Glycosidase;Host nucleus;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal;Virulence;Xylan degradation |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:34233072}. Host nucleus {ECO:0000269|PubMed:34233072}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..19; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 23,968 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |