Detail Information for IndEnz0004000026
IED ID IndEnz0004000026
Enzyme Type ID xylanase000026
Protein Name Endo-1,4-beta-xylanase D
Xylanase D
EC 3.2.1.8
1,4-beta-D-xylan xylanohydrolase D
Gene Name XYLD FGRRES_11304 FGSG_11304
Organism Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Sordariomycetes Hypocreomycetidae Hypocreales Nectriaceae Fusarium Fusarium sambucinum species complex Gibberella zeae (Wheat head blight fungus) (Fusarium graminearum) Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) (Wheat head blight fungus) (Fusarium graminearum)
Enzyme Sequence MHFLGLVVAFAPAALAQSAIWGQCGGTGWSGSTTCQSGLKCEKINDFYYQCIPGSDNGGGTTPDPGTPSPGNGNADATGLDAKIRAKGKIYFGTEIDHYHLSNNPLINIVKKDFGQVTNENSMKWDAIEPSRGQFTFSNADKVVDFAQANGKKIRGHTLLWYSQLPQWVKNIRDRATMTSVIENHVKTVVTRYKGKILHWDVVNEIFAEDGNMRNSEFYQVLGEDFVGIAFRAARAADPAAKLYINDYNLDIANYAKVTRGMVDHVNKWVSQGIPIDGIGSQAHLAKPGGWNPASGFPAALKVLAGANVKEVAITELDIDGAAANDYVTVVNSCLTTPKCVGITVWGVSDKDSWRSESNPLLFDRNYQPKAAYTAVSNALN
Enzyme Length 381
Uniprot Accession Number I1S3C6
Absorption
Active Site ACT_SITE 205; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 316; /note=Nucleophile; /evidence=ECO:0000250
Activity Regulation ACTIVITY REGULATION: Inhibited by the wheat xylanase inhibiting protein I (XIP-I). {ECO:0000269|Ref.7}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|PubMed:18707875, ECO:0000269|Ref.7};
DNA Binding
EC Number 3.2.1.8
Enzyme Function FUNCTION: Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Plays an important role in causing fusarium head blight (FHB) on cereal crops. {ECO:0000269|PubMed:18707875, ECO:0000269|Ref.7}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:18707875, ECO:0000269|Ref.7};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0. {ECO:0000269|PubMed:18707875, ECO:0000269|Ref.7};
Pathway PATHWAY: Glycan degradation; xylan degradation.
nucleotide Binding
Features Active site (2); Chain (1); Compositional bias (1); Disulfide bond (1); Domain (2); Region (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Secreted;Signal;Virulence;Xylan degradation
Interact With
Induction INDUCTION: Expression is under the control of transcription factor XYR1 and highly induced by xylan, carboxymethylcellulose (CMC), and hop cell wall. {ECO:0000269|PubMed:16707104, ECO:0000269|PubMed:17924109, ECO:0000269|PubMed:23337356}.
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..18; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 41,327
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda