Detail Information for IndEnz0004000034
IED ID IndEnz0004000034
Enzyme Type ID xylanase000034
Protein Name Ethylene-responsive transcription factor 4
Ethylene-responsive element-binding factor 3
EREBP-3
Ethylene-responsive element-binding factor 4 homolog
NtERF3
Gene Name ERF4 ERF-3 ERF3
Organism Nicotiana tabacum (Common tobacco)
Taxonomic Lineage cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae asterids lamiids Solanales Solanaceae Nicotianoideae Nicotianeae Nicotiana Nicotiana tabacum (Common tobacco)
Enzyme Sequence MAVKNKVSNGNLKGGNVKTDGVKEVHYRGVRKRPWGRYAAEIRDPGKKSRVWLGTFDTAEEAAKAYDTAAREFRGPKAKTNFPSPTENQSPSHSSTVESSSGENGVHAPPHAPLELDLTRRLGSVAADGGDNCRRSGEVGYPIFHQQPTVAVLPNGQPVLLFDSLWRAGVVNRPQPYHVTPMGFNGVNAGVGPTVSDSSSAVEENQYDGKRGIDLDLNLAPPMEF
Enzyme Length 225
Uniprot Accession Number Q40477
Absorption
Active Site
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity
DNA Binding DNA_BIND 26..83; /note=AP2/ERF; /evidence=ECO:0000255|PROSITE-ProRule:PRU00366
EC Number
Enzyme Function FUNCTION: Acts as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways mediated by ethylene, that seems to depend on a protein kinase/phosphatase cascade, and to be influenced by methyl-jasmonate. May regulate other AtERFs (By similarity). {ECO:0000250, ECO:0000269|PubMed:10652129, ECO:0000269|PubMed:10792818, ECO:0000269|PubMed:12654868, ECO:0000269|PubMed:7756828, ECO:0000269|Ref.2}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Chain (1); Compositional bias (1); DNA binding (1); Motif (1); Region (3)
Keywords DNA-binding;Ethylene signaling pathway;Nucleus;Plant defense;Reference proteome;Repressor;Transcription;Transcription regulation;Ubl conjugation
Interact With
Induction INDUCTION: Strongly induced by ethephon (ethylene-releasing compound) in buds and to lower extent in leaves. Strongly induced by cycloheximide and mechanical stimuli. Wounding leads to a both local and systemical transient expression, independently of ethylene, and through a de-novo-protein-synthesis-independent regulation. Not influenced by methyl-jasmonate. Induction by purified xylanase from Trichoderma viride (TvX) and another elicitor from Phytophthora infestans (PiE), that appears to be mediated by a protein kinase cascade, and to be negatively regulated by protein phosphatases. {ECO:0000269|PubMed:10652129, ECO:0000269|PubMed:12090623, ECO:0000269|PubMed:7756828, ECO:0000269|Ref.2}.
Subcellular Location SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00366, ECO:0000269|PubMed:10792818}.
Modified Residue
Post Translational Modification PTM: Probably negatively regulated by UBC2, that targets it to the ubiquitin proteasome pathway after ubiquitination and leads it to proteolysis. {ECO:0000305}.
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID 11487705; 23629833; 25996234;
Motif MOTIF 215..221; /note=EAR-like (transcriptional repression); /evidence=ECO:0000255
Gene Encoded By
Mass 24,262
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda