| IED ID | IndEnz0004000038 |
| Enzyme Type ID | xylanase000038 |
| Protein Name |
DNA gyrase subunit A EC 5.6.2.2 |
| Gene Name | gyrA Fisuc_0363 FSU_0778 |
| Organism | Fibrobacter succinogenes (strain ATCC 19169 / S85) |
| Taxonomic Lineage | cellular organisms Bacteria FCB group Fibrobacteres Fibrobacteria Fibrobacterales Fibrobacteraceae Fibrobacter Fibrobacter succinogenes (Bacteroides succinogenes) Fibrobacter succinogenes subsp. succinogenes Fibrobacter succinogenes (strain ATCC 19169 / S85) |
| Enzyme Sequence | MSEEMVPGSQFKSLVEQDMQDCYLRYSMSVIVARALPDARDGFKPVHRRVMYSMHKLGVVPNKGTVKSARIVGDVIGKYHPHGDVAVYDTLARMAQDFSLRYPLVFGQGNFGSIDGDSPAAMRYTEAKMNNLGALMLEDLEKETVDMGPNYDESLEEPLVLPSALPNMIVNGTSGIAVGMATNMAPHNLREVGAAIHALAENPDLTGEDLMEYVKGPDFPTGAIVCGRSGIREAYLTGHGRVRVRARTEIETDAKGKPRIIVTEIPYMVNKAELCKKIADLVRDKRIDGITDIRDESSRDIRIVIELRRDAVGEVVLNNLFKYTQLQTTFSIYNLALVNNLPKLLTLKDLLQVYIDHRLDVITRATQFDLKKAAARLHIIEGLRIATQNIDEVVKIIRQSKTTEIAKQSLQDRFSLDEIQSQAIVDMRLAQLVGLNIEKLEAEYNELIATVADLKDILEKRERRVAIMLQKLDAVVDKYGDERRTTIGEAIDDSDDEDLIAEEEQVITLSKEGYIRRLPIDTFKAQNRGGKGIIGAGLKDEDNVEQIFTASTHSYLLVFTNKGRVYWTKVYRLPEGARNGKGRPIVNFVALTEGEKVQAIVPVRKFGGYFCLVFATKKGIINKMDLTLFSRPRKAGVNAISLDEDDELVKVQLVGMSAEEYEASKNASDDDSAEAVENAAEAQAAEAAIAEESESGDAEDFANRPIPKDLLMIATKNGQAVTFPISCFRAMGRGTHGVKGITLAEGDEVISLLWLKAGNKILTITEKGYGKRSEPGSYRVTRRGSKGVRNLNVTDKIGAAVFVESVADDYDLIITSKDGQVIRIKAADIRLTGRNAQGVKAITLRDGDVVKDATALPSVEDIEQDSADAKETFDKVKGVEVDDDSVVKDDAEKQEIGPTETEE |
| Enzyme Length | 903 |
| Uniprot Accession Number | P35810 |
| Absorption | |
| Active Site | ACT_SITE 124; /note=O-(5'-phospho-DNA)-tyrosine intermediate; /evidence=ECO:0000255|HAMAP-Rule:MF_01897 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP-dependent breakage, passage and rejoining of double-stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-Rule:MF_01897}; |
| DNA Binding | |
| EC Number | 5.6.2.2 |
| Enzyme Function | FUNCTION: A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner. {ECO:0000255|HAMAP-Rule:MF_01897}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Erroneous initiation (1); Motif (1); Region (1) |
| Keywords | ATP-binding;Cytoplasm;DNA-binding;Isomerase;Nucleotide-binding;Reference proteome;Topoisomerase |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01897}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 526..532; /note=GyrA-box; /evidence=ECO:0000255|HAMAP-Rule:MF_01897 |
| Gene Encoded By | |
| Mass | 99,633 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |