IED ID | IndEnz0004000042 |
Enzyme Type ID | xylanase000042 |
Protein Name |
Endoglucanase B EC 3.2.1.4 Cellulase B Endo-1,4-beta-glucanase B Endo-1,4-beta-xylanase EC 3.2.1.8 |
Gene Name | engB Clocel_1150 |
Organism | Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Clostridiaceae Clostridium Clostridium cellulovorans Clostridium cellulovorans (strain ATCC 35296 / DSM 3052 / OCM 3 / 743B) |
Enzyme Sequence | MNKRLSRGKISLLASVFVTTTFMGGVNVLASTAKTGIRDITSQQVVKEMKVGWNLGNTMDATGGETNWGNPLTTHAMIDKVKAAGFNTLRLPITWDGHIGAAPDYAIDATWMNRVEEIANYAFDNNMYVIINLHHEDGWLKPYYANEAEVKAKITKVWTQIANRFKDYGDYLIFETMNEPRPVGAADEWSGGSYENRDMVNRYNLTAVNTIRATGGNNALRHIMVPTLAAAALSTTMNDYIVPNNDSRVIVSLHMYSPYFFSADLTSQWTTATWGSDADKAALSADFDAVYNKFVKNGRAVVIGEMGTINKNNLDSRVKHAEYYAKEATVRGITPIWWDNGYCVAGKEQTFGIFNRKNLTWCCPEVMQAFIRGAGATQTQTSYSLGDVNKDGKVNAIDYAVLKSILLGTNTNVDLSVSDMNKDGKVNALDLAVLKKMLLS |
Enzyme Length | 440 |
Uniprot Accession Number | P28621 |
Absorption | |
Active Site | ACT_SITE 179; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 305; /note=Nucleophile; /evidence=ECO:0000250 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; |
DNA Binding | |
EC Number | 3.2.1.4; 3.2.1.8 |
Enzyme Function | FUNCTION: Has endoglucanase activity on carboxymethyl-cellulose (CMC), xylan and lichenan, but not Avicel. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (2); Chain (1); Domain (1); Signal peptide (1) |
Keywords | Carbohydrate metabolism;Cellulose degradation;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Signal |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..33; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | 17693494; |
Motif | |
Gene Encoded By | |
Mass | 48,637 |
Kinetics | |
Metal Binding | |
Rhea ID | |
Cross Reference Brenda |