| IED ID | IndEnz0004000045 |
| Enzyme Type ID | xylanase000045 |
| Protein Name |
Endoglucanase EC 3.2.1.4 Carboxymethyl-cellulase CMCase Cellulase Endo-1,4-beta-glucanase |
| Gene Name | eglS bglC gld BSU18130 |
| Organism | Bacillus subtilis (strain 168) |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168) |
| Enzyme Sequence | MKRSISIFITCLLITLLTMGGMIASPASAAGTKTPVAKNGQLSIKGTQLVNRDGKAVQLKGISSHGLQWYGEYVNKDSLKWLRDDWGITVFRAAMYTADGGYIDNPSVKNKVKEAVEAAKELGIYVIIDWHILNDGNPNQNKEKAKEFFKEMSSLYGNTPNVIYEIANEPNGDVNWKRDIKPYAEEVISVIRKNDPDNIIIVGTGTWSQDVNDAADDQLKDANVMYALHFYAGTHGQFLRDKANYALSKGAPIFVTEWGTSDASGNGGVFLDQSREWLKYLDSKTISWVNWNLSDKQESSSALKPGASKTGGWRLSDLSASGTFVRENILGTKDSTKDIPETPSKDKPTQENGISVQYRAGDGSMNSNQIRPQLQIKNNGNTTVDLKDVTARYWYKAKNKGQNFDCDYAQIGCGNVTHKFVTLHKPKQGADTYLELGFKNGTLAPGASTGNIQLRLHNDDWSNYAQSGDYSFFKSNTFKTTKKITLYDQGKLIWGTEPN |
| Enzyme Length | 499 |
| Uniprot Accession Number | P10475 |
| Absorption | |
| Active Site | ACT_SITE 169; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:O85465; ACT_SITE 257; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:O85465 |
| Activity Regulation | |
| Binding Site | BINDING 65; /note=Substrate; /evidence=ECO:0000250|UniProtKB:O85465; BINDING 96; /note=Substrate; /evidence=ECO:0000250|UniProtKB:O85465; BINDING 131; /note=Substrate; /evidence=ECO:0000250|UniProtKB:O85465; BINDING 231; /note=Substrate; /evidence=ECO:0000250|UniProtKB:O85465; BINDING 291; /note=Substrate; /evidence=ECO:0000250|UniProtKB:O85465 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4; |
| DNA Binding | |
| EC Number | 3.2.1.4 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (23); Binding site (5); Chain (1); Domain (1); Erroneous initiation (1); Helix (15); Region (3); Sequence conflict (1); Signal peptide (1); Turn (5) |
| Keywords | 3D-structure;Carbohydrate metabolism;Cellulose degradation;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Reference proteome;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..29; /evidence=ECO:0000269|PubMed:7710279 |
| Structure 3D | NMR spectroscopy (1); X-ray crystallography (5) |
| Cross Reference PDB | 2L8A; 3PZT; 3PZU; 3PZV; 6UFV; 6UFW; |
| Mapped Pubmed ID | 21880019; |
| Motif | |
| Gene Encoded By | |
| Mass | 55,287 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.4; |