Detail Information for IndEnz0004000047
IED ID IndEnz0004000047
Enzyme Type ID xylanase000047
Protein Name Beta-glucanase
EC 3.2.1.73
1,3-1,4-beta-D-glucan 4-glucanohydrolase
Endo-beta-1,3-1,4 glucanase
Lichenase
Gene Name bglS bgl licS BSU39070 N15B
Organism Bacillus subtilis (strain 168)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168)
Enzyme Sequence MPYLKRVLLLLVTGLFMSLFAVTATASAQTGGSFFDPFNGYNSGFWQKADGYSNGNMFNCTWRANNVSMTSLGEMRLALTSPAYNKFDCGENRSVQTYGYGLYEVRMKPAKNTGIVSSFFTYTGPTDGTPWDEIDIEFLGKDTTKVQFNYYTNGAGNHEKIVDLGFDAANAYHTYAFDWQPNSIKWYVDGQLKHTATNQIPTTPGKIMMNLWNGTGVDEWLGSYNGVNPLYAHYDWVRYTKK
Enzyme Length 242
Uniprot Accession Number P04957
Absorption
Active Site ACT_SITE 133; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10064; ACT_SITE 137; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10064
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds.; EC=3.2.1.73;
DNA Binding
EC Number 3.2.1.73
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (2); Beta strand (16); Chain (1); Disulfide bond (1); Domain (1); Helix (4); Modified residue (1); Natural variant (3); Signal peptide (1); Turn (1)
Keywords 3D-structure;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Pyrrolidone carboxylic acid;Reference proteome;Secreted;Signal
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue MOD_RES 29; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000269|Ref.7
Post Translational Modification
Signal Peptide SIGNAL 1..28; /evidence=ECO:0000269|Ref.7
Structure 3D X-ray crystallography (1)
Cross Reference PDB 3O5S;
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 27,269
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.2.1.73;