Detail Information for IndEnz0004000056
IED ID IndEnz0004000056
Enzyme Type ID xylanase000056
Protein Name Anti-sigma-I factor RsgI6
Endo-1,4-beta-xylanase
EC 3.2.1.8
Gene Name rsgI6 Cthe_2119
Organism Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Hungateiclostridium thermocellum)
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Firmicutes Clostridia Eubacteriales Oscillospiraceae Acetivibrio Acetivibrio thermocellus (Hungateiclostridium thermocellum) Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Hungateiclostridium thermocellum)
Enzyme Sequence MIVGKVLDMDEKTAIIMTDDFAFLNVVRTSEMAVGKKVKVLDSDIIKPKNSLRRYLPVAAVAACFVIVLSFVLMFINGNTARKNIYAYVGIDINPSIELWINYNNKIAEAKALNGDAETVLEGLELKEKTVAEAVNEIVQKSMELGFISREKENIILISTACDLKAGEGSENKDVQNKIGQLFDDVNKAVSDLKNSGITTRILNLTLEERESSKEENISMGRYAVYLKAKEQNVNLTIDEIKDADLLELIAKVGIDNENVPEDIVTEDKDNLDAINTGPAESAVPEVTETLPATSTPGRTEGNTATGSVDSTPALSKNETPGKTETPGRTFNTPAKSSLGQSSTPKPVSPVQTATATKGIGTLTPRNSPTPVIPSTGIQWIDQANERINEIRKRNVQIKVVDSSNKPIENAYVEAVLTNHAFGFGTAITRRAMYDSNYTKFIKDHFNWAVFENESKWYTNEPSMGIITYDDADYLYEFCRSNGIKVRGHCIFWEAEEWQPAWVRSLDPFTLRFAVDNRLNSAVGHFKGKFEHWDVNNEMIHGNFFKSRLGESIWPYMFNRAREIDPNAKYFVNNNITTLKEADDCVALVNWLRSQGVRVDGVGVHGHFGDSVDRNLLKGILDKLSVLNLPIWITEYDSVTPDEYRRADNLENLYRTAFSHPSVEGIVMWGFWERVHWRGRDASIVNDNWTLNEAGRRFESLMNEWTTRAYGSTDGSGSFGFRGFYGTYRITVTVPGKGKYNYTLNLNRGSGTLQTTYRIP
Enzyme Length 760
Uniprot Accession Number A3DH97
Absorption
Active Site ACT_SITE 538; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01096; ACT_SITE 635; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01096
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|PubMed:20820855};
DNA Binding
EC Number 3.2.1.8
Enzyme Function FUNCTION: Anti-sigma factor for SigI6. Negatively regulates SigI6 activity through direct interaction. Binding of the polysaccharide substrate to the extracellular C-terminal sensing domain of RsgI6 may induce a conformational change in its N-terminal cytoplasmic region, leading to the release and activation of SigI6 (By similarity). Binds to and hydrolyzes insoluble and soluble xylan substrates. Has low enzymatic activity (PubMed:20820855). {ECO:0000250|UniProtKB:A3DBH1, ECO:0000269|PubMed:20820855}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan degradation; xylan degradation. {ECO:0000255|PROSITE-ProRule:PRU01096}.
nucleotide Binding
Features Active site (2); Chain (1); Compositional bias (1); Domain (2); Region (1); Topological domain (2); Transmembrane (1)
Keywords Carbohydrate metabolism;Cell membrane;Glycosidase;Hydrolase;Membrane;Polysaccharide degradation;Reference proteome;Transmembrane;Transmembrane helix;Xylan degradation
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000255}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 85,123
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda