IED ID | IndEnz0004000061 |
Enzyme Type ID | xylanase000061 |
Protein Name |
DNA topoisomerase 4 subunit B EC 5.6.2.2 Topoisomerase IV subunit B |
Gene Name | parE grlB BSU18090 |
Organism | Bacillus subtilis (strain 168) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus subtilis group Bacillus subtilis Bacillus subtilis subsp. subtilis Bacillus subtilis (strain 168) |
Enzyme Sequence | MARKQQFDYNEDAIQVLEGLEAVRKRPGMYIGSTDARGLHHLVYEIVDNSVDEVLAGHGDHIIVKIHKDNSISVQDRGRGMPTGMHKLGKPTPEVILTVLHAGGKFGQGGYKTSGGLHGVGASVVNALSEWLTVTIERDGFVYQQRFENGGKPVTSLEKIGKTKKTGTLTHFKPDPTMFSTTTYNFETLSERLRESAFLLKGLKIELIDERNDQREVFYYENGIEAFVAYLNEEKDVLSEVVSFEGEHHSIEVDFAFQFNDGYSENILSFVNNVRTKDGGTHESGAKTAMTRAFNEYARKVALLKEKDKNLEGTDIREGLSAIISVRIPEELLQFEGQTKGKLGTSEARSAVDAIVSEQLAYFLEENRDTATLLVKKAIKASQAREAARKAREEARSGKKRKKSEATLSGKLTPAGSRNPAKNELYLVEGDSAGGSAKQGRDRRFQAVLPLRGKVINTEKAKLADIFKNEEINTIIHAIGGGVGADFSIDDINYDKIIIMTDADTDGAHIQVLLLTFFYRYMKPLIEHGKVFIALPPLYKVSKGSGKKEIIEYAWSDEEMGDVLKKVGKGYTIQRYKGLGEMNADQLWETTMNPESRTLVRVKIDDAARVERRVTTLMGDKVEPRRKWIEKNVAFGLDEESNILENENLSVAEEV |
Enzyme Length | 655 |
Uniprot Accession Number | Q59192 |
Absorption | |
Active Site | |
Activity Regulation | |
Binding Site | BINDING 9; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00939; BINDING 49; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00939; BINDING 76; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00939; BINDING 340; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00939 |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=ATP-dependent breakage, passage and rejoining of double-stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-Rule:MF_00939}; |
DNA Binding | |
EC Number | 5.6.2.2 |
Enzyme Function | FUNCTION: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule. {ECO:0000255|HAMAP-Rule:MF_00939}. |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | NP_BIND 116..122; /note=ATP; /evidence=ECO:0000255|HAMAP-Rule:MF_00939 |
Features | Binding site (4); Chain (1); Compositional bias (1); Domain (1); Erroneous initiation (1); Metal binding (4); Nucleotide binding (1); Region (1); Site (4) |
Keywords | ATP-binding;DNA-binding;Isomerase;Magnesium;Metal-binding;Nucleotide-binding;Reference proteome;Topoisomerase |
Interact With | |
Induction | |
Subcellular Location | |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 73,007 |
Kinetics | |
Metal Binding | METAL 429; /note=Magnesium 1; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_00939; METAL 502; /note=Magnesium 1; catalytic; /evidence=ECO:0000255|HAMAP-Rule:MF_00939; METAL 502; /note=Magnesium 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00939; METAL 504; /note=Magnesium 2; /evidence=ECO:0000255|HAMAP-Rule:MF_00939 |
Rhea ID | |
Cross Reference Brenda |