Detail Information for IndEnz0004000063
IED ID IndEnz0004000063
Enzyme Type ID xylanase000063
Protein Name Endo-1,4-beta-xylanase B
Xylanase B
EC 3.2.1.8
1,4-beta-D-xylan xylanohydrolase B
Gene Name xynB
Organism Penicillium oxalicum
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Aspergillaceae Penicillium Penicillium oxalicum
Enzyme Sequence MISLSSVAIALTTVVGALALPSDQSVNLAARQAITSSQTGTNNGYYYSFWTNGAGSVSYSNGAAGQFSVNWANQGGGDFTCGKGWNPGKAQDISFSGTFTPNGNAYLSIYGWTTGPLVEYYILENFGSYNPGNGMTHVGTLTSDGSDYDIYKHTQVNQPSIVGTSTFDQYWSIRKNKRSSGTVTTANHFSAWASHGMNLGSHNYQILSVEGYQSSGSASMTVSAGSSSSGGSGSGSGSGSGSGSGSGSQTTTAGSSTGTGTGSGSGSGSGGSGGNCAAQWGQCGGQGWNGPTCCSSGTCKASNQWYSQCL
Enzyme Length 310
Uniprot Accession Number E7EF85
Absorption
Active Site ACT_SITE 119; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10062; ACT_SITE 210; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10063
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069};
DNA Binding
EC Number 3.2.1.8
Enzyme Function FUNCTION: Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Hydrolyzes birchwood xylan, beechwood xylan, and oat spelt xylan to produce short-chain xylooligosaccharides, xylopentaose, xylotriose, and xylobiose as the main products. {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}.
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 50 degrees Celsius. {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069};
PH Dependency BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5.0. {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069};
Pathway PATHWAY: Glycan degradation; xylan degradation.
nucleotide Binding
Features Active site (2); Chain (1); Domain (2); Region (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal;Xylan degradation
Interact With
Induction INDUCTION: Induced by soluble xylo-oligosaccharides, with beechwood xylan being the best inducer. {ECO:0000269|PubMed:22940308}.
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23412069}.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 31,282
Kinetics BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=5 mg/ml for birchwood xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; KM=4.6 mg/ml for beechwood xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; KM=11.3 mg/ml for oat spelt xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; Vmax=2 umol/min/mg enzyme toward birchwood xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; Vmax=2 umol/min/mg enzyme toward beechwood xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069}; Vmax=2.5 umol/min/mg enzyme toward oat spelt xylan {ECO:0000269|PubMed:22940308, ECO:0000269|PubMed:23412069};
Metal Binding
Rhea ID
Cross Reference Brenda 3.2.1.8;