Detail Information for IndEnz0004000064
IED ID IndEnz0004000064
Enzyme Type ID xylanase000064
Protein Name Endo-1,4-beta-xylanase B
Xylanase B
EC 3.2.1.8
1,4-beta-D-xylan xylanohydrolase B
Gene Name xlnB
Organism Streptomyces lividans
Taxonomic Lineage cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces Streptomyces lividans
Enzyme Sequence MNLLVQPRRRRRGPVTLLVRSAWAVALAALAALMLPGTAQADTVVTTNQEGTNNGYYYSFWTDSQGTVSMNMGSGGQYSTSWRNTGNFVAGKGWANGGRRTVQYSGSFNPSGNAYLALYGWTSNPLVEYYIVDNWGTYRPTGEYKGTVTSDGGTYDIYKTTRVNKPSVEGTRTFDQYWSVRQSKRTGGTITTGNHFDAWARAGMPLGNFSYYMIMATEGYQSSGSSSINVGGTGGGDSGGGDNGGGGGGCTATVSAGQKWGDRYNLDVSVSGASDWTVTMNVPSPAKVLSNWNVNASYPSAQTLTARLNGSGNNWGATIQANANWTWPSVSCSAG
Enzyme Length 335
Uniprot Accession Number P26515
Absorption
Active Site ACT_SITE 128; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10062; ACT_SITE 218; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10063
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8;
DNA Binding
EC Number 3.2.1.8
Enzyme Function FUNCTION: Contributes to hydrolyze hemicellulose, the major component of plant cell-walls. XLNA and XLNB seem to act sequentially on the substrate to yield xylobiose and xylose as carbon sources.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan degradation; xylan degradation.
nucleotide Binding
Features Active site (2); Beta strand (12); Chain (1); Domain (2); Helix (1); Region (2); Signal peptide (1); Turn (1)
Keywords 3D-structure;Carbohydrate metabolism;Direct protein sequencing;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal;Xylan degradation
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..41; /evidence=ECO:0000269|PubMed:1743521
Structure 3D X-ray crystallography (1)
Cross Reference PDB 5EJ3;
Mapped Pubmed ID 27387012;
Motif
Gene Encoded By
Mass 35,575
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda