Detail Information for IndEnz0004000067
IED ID IndEnz0004000067
Enzyme Type ID xylanase000067
Protein Name Endo-1,4-beta-xylanase 11A
Xylanase 11A
EC 3.2.1.8
1,4-beta-D-xylan xylanohydrolase 11A
Gene Name xyn11A
Organism Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta sordariomyceta Leotiomycetes Helotiales Sclerotiniaceae Botrytis Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)
Enzyme Sequence MVSASSLLLAASAIAGVFSAPAAAPVSENLNVLQERALTSSATGTSGGYYYSFWTDGSGGVTYSNGANGQYAVSWTGNKGNFVGGKGWAVGSERSISYTGSYKPNGNSYLSVYGWTTSPLIEYYIVEDFGTYDPSSAATEIGSVTSDGSTYKILETTRTNQPSVQGTATFKQYWSVRTSKRTSGTVTTANHFAAWKKLGLTLGSTYNYQIVAVEGYQSSGSASITVS
Enzyme Length 227
Uniprot Accession Number Q2LMP0
Absorption
Active Site ACT_SITE 122; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10062; ACT_SITE 214; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10063
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8;
DNA Binding
EC Number 3.2.1.8
Enzyme Function FUNCTION: Endo-1,4-beta-xylanase involved in the hydrolysis of xylan, a major structural heterogeneous polysaccharide found in plant biomass representing the second most abundant polysaccharide in the biosphere, after cellulose. Required for planr infection and the appearance of secondary lesions. {ECO:0000269|PubMed:16404950}.
Temperature Dependency
PH Dependency
Pathway PATHWAY: Glycan degradation; xylan degradation.
nucleotide Binding
Features Active site (2); Chain (1); Domain (1); Signal peptide (1)
Keywords Carbohydrate metabolism;Glycosidase;Hydrolase;Polysaccharide degradation;Secreted;Signal;Virulence;Xylan degradation
Interact With
Induction INDUCTION: Expression is maximal when xylan is the only carbon source, and repressed by glucose. Expression is detected from the beginning of tomato leaves infection in the just-inoculated leaves, and the level sof transcript increased between 24 and 48 hours postinfection simultaneously with the appearance of visible lesions on the leaves. {ECO:0000269|PubMed:16404950}.
Subcellular Location SUBCELLULAR LOCATION: Secreted.
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..19; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 23,824
Kinetics
Metal Binding
Rhea ID
Cross Reference Brenda 3.2.1.8;