| IED ID | IndEnz0004000087 |
| Enzyme Type ID | xylanase000087 |
| Protein Name |
Endo-1,4-beta-xylanase A Xylanase A EC 3.2.1.8 |
| Gene Name | xynAS9 |
| Organism | Streptomyces sp. |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Streptomycetales Streptomycetaceae Streptomyces unclassified Streptomyces Streptomyces sp. |
| Enzyme Sequence | MFRHHPTRGRRTAGLLAAALATLSAGLTAVAPAHPARADTATLGELAEAKGRYFGSATDNPELPDTQYTQILGSEFSQITVGNTMKWQYTEPSRGRFDYTAAEEIVDLAESNGQSVRGHTLVWHNQLPSWVDDVPAGELLGVMRDHITHEVDHFKGRLIHWDVVNEAFEEDGSRRQSVFQQKIGDSYIAEAFKAARAADPDVKLYYNDYNIEGIGPKSDAVYEMVKSFKAQGIPIDGVGMQAHLIAGQVPASLQENIRRFADLGVDVALTELDIRMTLPRTAAKDAQQATDYGAVVEACLVVSRCVGITVWDYTDKYSWVPSVFPGQGAALPWDEDFAKKPAYHAIAAALNGGSPAPGGNCTATYRVTSQWQGGFTAEITVGNDHTAPITGWTVTWTLSSGQSISHMWNGNLTVNGQDVTVRDVGYNGTLGGNGSTTFGFQGEGVADTPADVTCTPGRPSGTSA |
| Enzyme Length | 464 |
| Uniprot Accession Number | B4XVN1 |
| Absorption | |
| Active Site | ACT_SITE 166; /note=Proton donor; /evidence=ECO:0000250; ACT_SITE 271; /note=Nucleophile; /evidence=ECO:0000250 |
| Activity Regulation | ACTIVITY REGULATION: Completely inhibited by Hg(2+), unaffected by EDTA. {ECO:0000269|PubMed:18521591}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; |
| DNA Binding | |
| EC Number | 3.2.1.8 |
| Enzyme Function | FUNCTION: Contributes to hydrolysis of hemicellulose, the major component of plant cell-walls. Hydrolyzes xylan to xylose and xylobiose. {ECO:0000269|PubMed:18521591}. |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 degrees Celsius, more than 80% of activity remains after 1 hour at 60 degrees Celsius. {ECO:0000269|PubMed:18521591}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.5, more than 80% active between pH 5.0 and 7.0. {ECO:0000269|PubMed:18521591}; |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (13); Chain (1); Domain (2); Helix (16); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Carbohydrate metabolism;Glycosidase;Hydrolase;Polysaccharide degradation;Signal;Xylan degradation |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..33; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (4) |
| Cross Reference PDB | 3WUB; 3WUE; 3WUF; 3WUG; |
| Mapped Pubmed ID | 25193708; |
| Motif | |
| Gene Encoded By | |
| Mass | 49,868 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: Vmax=772.20 umol/min/mg enzyme for oat spelt xylan {ECO:0000269|PubMed:18521591}; Vmax=490.87 umol/min/mg enzyme for birchwood xylan {ECO:0000269|PubMed:18521591}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.8; |