| IED ID | IndEnz0004000171 |
| Enzyme Type ID | xylanase000171 |
| Protein Name |
Endo-1,4-beta-xylanase Xylanase EC 3.2.1.8 1,4-beta-D-xylan xylanohydrolase |
| Gene Name | XYNA |
| Organism | Thermomyces lanuginosus (Humicola lanuginosa) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Pezizomycotina leotiomyceta Eurotiomycetes Eurotiomycetidae Eurotiales (green and blue molds) Trichocomaceae Thermomyces Thermomyces lanuginosus (Humicola lanuginosa) |
| Enzyme Sequence | MVGFTPVALAALAATGALAFPAGNATELEKRQTTPNSEGWHDGYYYSWWSDGGAQATYTNLEGGTYEISWGDGGNLVGGKGWNPGLNARAIHFEGVYQPNGNSYLAVYGWTRNPLVEYYIVENFGTYDPSSGATDLGTVECDGSIYRLGKTTRVNAPSIDGTQTFDQYWSVRQDKRTSGTVQTGCHFDAWARAGLNVNGDHYYQIVATEGYFSSGYARITVADVG |
| Enzyme Length | 225 |
| Uniprot Accession Number | O43097 |
| Absorption | |
| Active Site | ACT_SITE 117; /note=Nucleophile; ACT_SITE 209; /note=Proton donor |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|Ref.3}; |
| DNA Binding | |
| EC Number | 3.2.1.8 |
| Enzyme Function | |
| Temperature Dependency | BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Optimum temperature is 60 degrees Celsius. Thermostable. {ECO:0000269|Ref.3}; |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 6.0. {ECO:0000269|Ref.3}; |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (14); Chain (1); Disulfide bond (1); Domain (1); Helix (1); Modified residue (1); Sequence conflict (2); Signal peptide (1); Turn (2) |
| Keywords | 3D-structure;Carbohydrate metabolism;Direct protein sequencing;Disulfide bond;Glycosidase;Hydrolase;Polysaccharide degradation;Pyrrolidone carboxylic acid;Signal;Xylan degradation |
| Interact With | |
| Induction | INDUCTION: By xylan. {ECO:0000269|Ref.3}. |
| Subcellular Location | |
| Modified Residue | MOD_RES 32; /note=Pyrrolidone carboxylic acid; /evidence=ECO:0000250|UniProtKB:P36217 |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..31 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 1YNA; |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 24,356 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: Vmax=2223.3 umol/min/mg enzyme {ECO:0000269|Ref.3}; |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.8; |