| IED ID | IndEnz0004000212 |
| Enzyme Type ID | xylanase000212 |
| Protein Name |
Bifunctional xylanase/deacetylase Includes: Endo-1,4-beta-xylanase D XYLD Xylanase D EC 3.2.1.8 ; Acetylated xylan deacetylase EC 3.5.1.- |
| Gene Name | xynD |
| Organism | Cellulomonas fimi |
| Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Actinobacteria Actinomycetia (high G+C Gram-positive bacteria) Micrococcales Cellulomonadaceae Cellulomonas Cellulomonas fimi |
| Enzyme Sequence | MSDSFEATRTTRRRRPLQALTGLLAAGALVAGALAAASPAAAAVTSNTTGTHDGYFYSFWTDSPGSVSMDLNSGGGYTRWSNTGNFVAGKGWSTGGRKTVSYSGQFNPSRNAYLTLYGWTQSPLVEYYIVDSWGTYRPTGTFMGTVTSDGGTYDIYRTQRVNKPSIEGDSSTFYQYWSVRQQKRTGGTITSGNHFDAWASKGMNLGRHNYMIMATEGYQSSGSSSITVSEGSGGGGGGDTGGGGGSTGCSVTATRAEEWSDRFNVTYSVSGSSAWTVNLALNGSQTIQASWNANVTGSGSTRTVTPNGSGNTFGVTVMKNGSSTTPAATCAGSGGGTATPTPTPTPTPTPQSCSAGYVGLTFDDGPNTGTTNQILSTLTQYGATATVFPTGQNAQGNPSLMQAYKNAGVQIGNHSWDHPHLVNMSQSDMQSQLTRTQQAIQQTAGVTPTLFRPPYGESNATLRQVESSLGLREIIWDVDSQDWNNASASQIRQAASRLTNGQIILMHDWPAATVQALPGILQDLRSRNLCTGHISSSTGRAVAPSSAGGGGGGGGGTGSCSVSAVRGEEWADRFNVTYSVSGSSSWVVTLGLNGGQSVQSSWNAALTGSSGTVTARPNGSGNSFGVTFYKNGSSATPGATCATG |
| Enzyme Length | 644 |
| Uniprot Accession Number | P54865 |
| Absorption | |
| Active Site | ACT_SITE 126; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10062; ACT_SITE 216; /note=Proton donor; /evidence=ECO:0000255|PROSITE-ProRule:PRU10063 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; |
| DNA Binding | |
| EC Number | 3.2.1.8; 3.5.1.- |
| Enzyme Function | FUNCTION: Endo-acting xylanase which displays no detectable activity against polysaccharides other than xylan. Hydrolyzes glucosidic bonds with retention of anomeric configuration. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycan degradation; xylan degradation. |
| nucleotide Binding | |
| Features | Active site (2); Beta strand (16); Chain (1); Domain (4); Region (6); Signal peptide (1) |
| Keywords | 3D-structure;Carbohydrate metabolism;Glycosidase;Hydrolase;Multifunctional enzyme;Polysaccharide degradation;Repeat;Signal;Xylan degradation |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..43; /evidence=ECO:0000255 |
| Structure 3D | NMR spectroscopy (6) |
| Cross Reference PDB | 1E5B; 1E5C; 1HEH; 1HEJ; 1XBD; 2XBD; |
| Mapped Pubmed ID | 10425686; 10973978; 11327868; |
| Motif | |
| Gene Encoded By | |
| Mass | 66,582 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.2.1.8; |