Detail Information for IndEnz0005000027
IED ID IndEnz0005000027
Enzyme Type ID lipase000027
Protein Name Phosphatidylcholine-sterol acyltransferase
EC 2.3.1.43
Lecithin-cholesterol acyltransferase
Phospholipid-cholesterol acyltransferase
Fragment
Gene Name LCAT
Organism Gerbilliscus gambianus (Gambian gerbil) (Gerbilliscus kempi gambiana)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Deuterostomia Chordata Craniata Vertebrata Gnathostomata (jawed vertebrates) Teleostomi Euteleostomi Sarcopterygii Dipnotetrapodomorpha Tetrapoda Amniota Mammalia Theria Eutheria Boreoeutheria Euarchontoglires Glires (Rodents and rabbits) Rodentia Myomorpha (mice and others) Muroidea Muridae Gerbillinae (gerbils) Gerbilliscus Gerbilliscus gambianus (Gambian gerbil) (Gerbilliscus kempi gambiana)
Enzyme Sequence TIWLDLNMFLSLGVDCWIDNTRVVYNRSSGRVSNAPGVEIRVPGFGKTYSVEYLDDNKLAEYMHTLVQNLVNNGYVRDETVRAAPYDWRLEPSQQDDYYQKLAGLIEEMYAAYGKPVFLIGHSLGCLHVLYFLLRQGIPIMSSIKLREEQRITTTSPWMFPDRDVWPEDHVFISTPEFNYTGQDFERFFSDLHFEEGWYMWLQSRDLLAGLPAPGVDVYCLYGVGLPTPHTYIYDHNFPYKDPVAALYEDGDDTVATRSTELCGQWQGRQSQPVHLLPMNGTEHLNMVFSNKT
Enzyme Length 293
Uniprot Accession Number O35840
Absorption
Active Site ACT_SITE 123; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P04180; ACT_SITE 252; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P04180; ACT_SITE 284; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:P04180
Activity Regulation ACTIVITY REGULATION: APOA1 is the most potent activator in plasma. Also activated by APOE, APOC1 and APOA4 (By similarity). {ECO:0000250}.
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + a sterol = a 1-acyl-sn-glycero-3-phosphocholine + a sterol ester; Xref=Rhea:RHEA:21204, ChEBI:CHEBI:15889, ChEBI:CHEBI:35915, ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=2.3.1.43; Evidence={ECO:0000250|UniProtKB:P04180, ECO:0000255|PROSITE-ProRule:PRU10037};
DNA Binding
EC Number 2.3.1.43
Enzyme Function FUNCTION: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (By similarity). {ECO:0000250|UniProtKB:P04180}.
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (1); Glycosylation (3); Non-terminal residue (2); Site (1)
Keywords Acyltransferase;Cholesterol metabolism;Disulfide bond;Glycoprotein;Lipid metabolism;Secreted;Steroid metabolism;Sterol metabolism;Transferase
Interact With
Induction
Subcellular Location SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P04180}. Note=Secreted into blood plasma. Produced in astrocytes and secreted into cerebral spinal fluid (CSF) (By similarity). {ECO:0000250|UniProtKB:P04180}.
Modified Residue
Post Translational Modification
Signal Peptide
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 33,822
Kinetics
Metal Binding
Rhea ID RHEA:21204
Cross Reference Brenda