| IED ID | IndEnz0005000031 |
| Enzyme Type ID | lipase000031 |
| Protein Name |
Poly 3-hydroxyalkanoate depolymerase C PHA depolymerase EC 3.1.1.- PHB depolymerase |
| Gene Name | phaZ1 |
| Organism | Paucimonas lemoignei (Pseudomonas lemoignei) |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Betaproteobacteria Burkholderiales Burkholderiaceae Paucimonas Paucimonas lemoignei (Pseudomonas lemoignei) |
| Enzyme Sequence | MLAKQIKKANSRSTLLRKSLLFAAPIILAVSSSSVYALTQVSNFGTNPGNLQMFKHVPSGMPANAPLVVALHGCTQTAAAYEASGWSALGNTHKFYVVYPQQQSGNNSNKCFNWFEPGDITRGQGEALSIKQMVDNMKANHSIDPSRVYVTGLSAGAFMTTVMAATYPDVFAGAAPIAGGPYKCATSMTSAFTCMSPGVDKTPAAWGDLARGGYSGYNGPKPKISIWHGSSDYTVAPANQNETVEQFTNYHGIDQTPDVSDTVGGFPHKVYKSANGTPLVETYTITGMGHGTPVDPGTGANQCGTAGAYILDVNVCSSYYIGQFFGIIGGGGTTTTTTSGNVTTTTAATTTTTTATQGYTQTTSATVTNHYVAGRINVTQYNVLGARYGYVTTIPLYYCPSLSGWTDKANCSPI |
| Enzyme Length | 414 |
| Uniprot Accession Number | P52090 |
| Absorption | |
| Active Site | ACT_SITE 154; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P-nitrophenylbutyrate as substrates. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (1); Chain (1); Signal peptide (1) |
| Keywords | Hydrolase;Lipid degradation;Lipid metabolism;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..37; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,496 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.1.1.75; |