| IED ID | IndEnz0005000043 |
| Enzyme Type ID | lipase000043 |
| Protein Name |
Phospholipid:diacylglycerol acyltransferase 1 AtPDAT EC 2.3.1.158 |
| Gene Name | PDAT1 PDAT At5g13640 MSH12.10 T6I14.2 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MPLIHRKKPTEKPSTPPSEEVVHDEDSQKKPHESSKSHHKKSNGGGKWSCIDSCCWFIGCVCVTWWFLLFLYNAMPASFPQYVTERITGPLPDPPGVKLKKEGLKAKHPVVFIPGIVTGGLELWEGKQCADGLFRKRLWGGTFGEVYKRPLCWVEHMSLDNETGLDPAGIRVRAVSGLVAADYFAPGYFVWAVLIANLAHIGYEEKNMYMAAYDWRLSFQNTEVRDQTLSRMKSNIELMVSTNGGKKAVIVPHSMGVLYFLHFMKWVEAPAPLGGGGGPDWCAKYIKAVMNIGGPFLGVPKAVAGLFSAEAKDVAVARAIAPGFLDTDIFRLQTLQHVMRMTRTWDSTMSMLPKGGDTIWGGLDWSPEKGHTCCGKKQKNNETCGEAGENGVSKKSPVNYGRMISFGKEVAEAAPSEINNIDFRGAVKGQSIPNHTCRDVWTEYHDMGIAGIKAIAEYKVYTAGEAIDLLHYVAPKMMARGAAHFSYGIADDLDDTKYQDPKYWSNPLETKLPNAPEMEIYSLYGVGIPTERAYVYKLNQSPDSCIPFQIFTSAHEEDEDSCLKAGVYNVDGDETVPVLSAGYMCAKAWRGKTRFNPSGIKTYIREYNHSPPANLLEGRGTQSGAHVDIMGNFALIEDIMRVAAGGNGSDIGHDQVHSGIFEWSERIDLKL |
| Enzyme Length | 671 |
| Uniprot Accession Number | Q9FNA9 |
| Absorption | |
| Active Site | ACT_SITE 254; /note=Acyl-ester intermediate; /evidence=ECO:0000255; ACT_SITE 573; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 626; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycerol + a glycerophospholipid = a monoacylglycerophospholipid + a triacyl-sn-glycerol; Xref=Rhea:RHEA:14057, ChEBI:CHEBI:17815, ChEBI:CHEBI:64615, ChEBI:CHEBI:136912, ChEBI:CHEBI:136913; EC=2.3.1.158; Evidence={ECO:0000250|UniProtKB:O94680}; |
| DNA Binding | |
| EC Number | 2.3.1.158 |
| Enzyme Function | FUNCTION: Triacylglycerol formation by an acyl-CoA independent pathway. The enzyme preferentially transfers acyl groups from the sn-2 position of a phospholipid to diacylglycerol, thus forming an sn-1-lysophospholipid. Involved in epoxy and hydroxy fatty acid accumulation in seeds. Has complementary functions with DAG1 that are essential for triacylglycerol synthesis and normal development of both seeds and pollen. {ECO:0000269|PubMed:15247387, ECO:0000269|PubMed:20040537, ECO:0000269|PubMed:20101470}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Compositional bias (1); Erroneous initiation (1); Frameshift (1); Glycosylation (4); Region (1); Sequence conflict (1); Topological domain (2); Transmembrane (1) |
| Keywords | Acyltransferase;Glycerol metabolism;Glycoprotein;Lipid biosynthesis;Lipid metabolism;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14690501; 15539469; 15937229; 16020547; 16618929; 18667721; 18775970; 21173026; 21515696; 22233193; 23505340; 23824186; 24076979; 24118513; 25293755; 28733391; 29660088; 30537885; 31221730; 31319458; 32538516; 32732347; |
| Motif | |
| Gene Encoded By | |
| Mass | 74,157 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:14057 |
| Cross Reference Brenda | 2.3.1.158;2.3.1.20; |