| IED ID | IndEnz0005000044 |
| Enzyme Type ID | lipase000044 |
| Protein Name |
Putative phospholipid:diacylglycerol acyltransferase 2 AtPDAT2 EC 2.3.1.158 |
| Gene Name | PDAT2 At3g44830 F28D10.20 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MSPLLRFRKLSSFSEDTINPKPKQSATVEKPKRRRSGRCSCVDSCCWLIGYLCTAWWLLLFLYHSVPVPAMLQAPESPGTRLSRDGVKAFHPVILVPGIVTGGLELWEGRPCAEGLFRKRLWGASFSEILRRPLCWLEHLSLDSETGLDPSGIRVRAVPGLVAADYFAPCYFAWAVLIENLAKIGYEGKNLHMASYDWRLSFHNTEVRDQSLSRLKSKIELMYATNGFKKVVVVPHSMGAIYFLHFLKWVETPLPDGGGGGGPGWCAKHIKSVVNIGPAFLGVPKAVSNLLSAEGKDIAYARSLAPGLLDSELLKLQTLEHLMRMSHSWDSIVSLLPKGGEAIWGDLDSHAEEGLNCIYSKRKSSQLSLSNLHKQNYSLKPVSRVKEPAKYGRIVSFGKRASELPSSQLSTLNVKELSRVDGNSNDSTSCGEFWSEYNEMSRESIVKVAENTAYTATTVLDLLRFIAPKMMRRAEAHFSHGIADDLDDPKYGHYKYWSNPLETKLPEAPEMEMYCLYGVGIPTERSYIYKLATSSGKCKSSIPFRIDGSLDGDDVCLKGGTRFADGDESVPVISAGFMCAKGWRGKTRFNPSGMDTFLREYKHKPPGSLLESRGTESGAHVDIMGNVGLIEDVLRIAAGASGQEIGGDRIYSDVMRMSERISIKL |
| Enzyme Length | 665 |
| Uniprot Accession Number | Q9FYC7 |
| Absorption | |
| Active Site | ACT_SITE 237; /note=Acyl-ester intermediate; /evidence=ECO:0000255; ACT_SITE 567; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 620; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycerol + a glycerophospholipid = a monoacylglycerophospholipid + a triacyl-sn-glycerol; Xref=Rhea:RHEA:14057, ChEBI:CHEBI:17815, ChEBI:CHEBI:64615, ChEBI:CHEBI:136912, ChEBI:CHEBI:136913; EC=2.3.1.158; Evidence={ECO:0000250|UniProtKB:O94680}; |
| DNA Binding | |
| EC Number | 2.3.1.158 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Transmembrane (1) |
| Keywords | Acyltransferase;Glycerol metabolism;Lipid biosynthesis;Lipid metabolism;Membrane;Reference proteome;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14690501; 16020547; 17420955; 22233193; 23505340; |
| Motif | |
| Gene Encoded By | |
| Mass | 73,653 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:14057 |
| Cross Reference Brenda | 2.3.1.158; |