| IED ID | IndEnz0005000045 |
| Enzyme Type ID | lipase000045 |
| Protein Name |
Phospholipid:diacylglycerol acyltransferase PDAT EC 2.3.1.158 Pombe LRO1 homolog 1 Triacylglycerol synthase TAG synthase |
| Gene Name | plh1 SPBC776.14 |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MASSKKSKTHKKKKEVKSPIDLPNSKKPTRALSEQPSASETQSVSNKSRKSKFGKRLNFILGAILGICGAFFFAVGDDNAVFDPATLDKFGNMLGSSDLFDDIKGYLSYNVFKDAPFTTDKPSQSPSGNEVQVGLDMYNEGYRSDHPVIMVPGVISSGLESWSFNNCSIPYFRKRLWGSWSMLKAMFLDKQCWLEHLMLDKKTGLDPKGIKLRAAQGFEAADFFITGYWIWSKVIENLAAIGYEPNNMLSASYDWRLSYANLEERDKYFSKLKMFIEYSNIVHKKKVVLISHSMGSQVTYYFFKWVEAEGYGNGGPTWVNDHIEAFINISGSLIGAPKTVAALLSGEMKDTAQLNQFSVYGLEKFFSRSERAMMVRTMGGVSSMLPKGGDVVWGNASWAPDDLNQTNFSNGAIIRYREDIDKDHDEFDIDDALQFLKNVTDDDFKVMLAKNYSHGLAWTEKEVLKNNEMPSKWINPLETSLPYAPDMKIYCVHGVGKPTERGYYYTNNPEGQPVIDSSVNDGTKVENGIVMDDGDGTLPILALGLVCNKVWQTKRFNPANTSITNYEIKHEPAAFDLRGGPRSAEHVDILGHSELNEIILKVSSGHGDSVPNRYISDIQEIINEINLDKPRN |
| Enzyme Length | 632 |
| Uniprot Accession Number | O94680 |
| Absorption | |
| Active Site | ACT_SITE 293; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q8NCC3; ACT_SITE 535; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q8NCC3; ACT_SITE 586; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q8NCC3 |
| Activity Regulation | |
| Binding Site | BINDING 136; /note=Substrate; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:Q8NCC3; BINDING 294; /note=Substrate; via amide nitrogen; /evidence=ECO:0000250|UniProtKB:Q8NCC3 |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycerol + a glycerophospholipid = a monoacylglycerophospholipid + a triacyl-sn-glycerol; Xref=Rhea:RHEA:14057, ChEBI:CHEBI:17815, ChEBI:CHEBI:64615, ChEBI:CHEBI:136912, ChEBI:CHEBI:136913; EC=2.3.1.158; Evidence={ECO:0000269|PubMed:12963726}; |
| DNA Binding | |
| EC Number | 2.3.1.158 |
| Enzyme Function | FUNCTION: Catalyzes triacylglycerol (TAG) formation by an acyl-CoA independent pathway. The enzyme specifically transfers acyl groups from the sn-2 position of a phospholipid to diacylglycerol (DAG), thus forming an sn-1-lysophospholipid. Plays a major role in triacylglycerol formation at log phase (PubMed:12963726, PubMed:26990381). Involved in lipid particle synthesis from the endoplasmic reticulum, promoting localized TAG production at discrete ER subdomains (PubMed:26990381, PubMed:28011631). {ECO:0000269|PubMed:12963726, ECO:0000269|PubMed:26990381, ECO:0000269|PubMed:28011631}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis. {ECO:0000305|PubMed:12963726}. |
| nucleotide Binding | |
| Features | Active site (3); Binding site (2); Chain (1); Compositional bias (1); Region (1); Topological domain (2); Transmembrane (1) |
| Keywords | Acyltransferase;Endoplasmic reticulum;Membrane;Reference proteome;Signal-anchor;Transferase;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:12963726, ECO:0000269|PubMed:16823372}; Single-pass type II membrane protein {ECO:0000255}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 20473289; 22633491; 23697806; 30726745; 34250083; |
| Motif | |
| Gene Encoded By | |
| Mass | 70,833 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:14057 |
| Cross Reference Brenda |