| IED ID | IndEnz0005000060 |
| Enzyme Type ID | lipase000060 |
| Protein Name |
Phospholipase A1 PLIP1, chloroplastic EC 3.1.1.32 Galactolipase PLIP1 EC 3.1.1.26 Protein PLASTID LIPASE 1 |
| Gene Name | PLIP1 At3g61680 F15G16.70 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAFNTAMASTSPAAANDVLREHIGLRRSLSGQDLVLKGGGIRRSSSDNHLCCRSGNNNNRILAVSVRPGMKTSRSVGVFSFQISSSIIPSPIKTLLFETDTSQDEQESDEIEIETEPNLDGAKKANWVERLLEIRRQWKREQKTESGNSDVAEESVDVTCGCEEEEGCIANYGSVNGDWGRESFSRLLVKVSWSEAKKLSQLAYLCNLAYTIPEIKGEDLRRNYGLKFVTSSLEKKAKAAILREKLEQDPTHVPVITSPDLESEKQSQRSASSSASAYKIAASAASYIHSCKEYDLSEPIYKSAAAAQAAASTMTAVVAAGEEEKLEAARELQSLQSSPCEWFVCDDPNTYTRCFVIQGSDSLASWKANLFFEPTKFEDTDVLVHRGIYEAAKGIYEQFLPEITEHLSRHGDRAKFQFTGHSLGGSLSLIVNLMLISRGLVSSEAMKSVVTFGSPFVFCGGEKILAELGLDESHVHCVMMHRDIVPRAFSCNYPDHVALVLKRLNGSFRTHPCLNKNKLLYSPMGKVYILQPSESVSPTHPWLPPGNALYILENSNEGYSPTALRAFLNRPHPLETLSQRAAYGSEGSVLRDHDSKNYVKAVNGVLRQHTKLIVRKARIQRRSVWPVLTSAGRGLNESLTTAEEIMTRV |
| Enzyme Length | 649 |
| Uniprot Accession Number | Q7Y220 |
| Absorption | |
| Active Site | ACT_SITE 422; /note=Acyl-ester intermediate; /evidence=ECO:0000250|UniProtKB:Q948R1; ACT_SITE 483; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1; ACT_SITE 593; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q948R1 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 2-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+); Xref=Rhea:RHEA:18689, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:28868, ChEBI:CHEBI:57643, ChEBI:CHEBI:57875; EC=3.1.1.32; Evidence={ECO:0000269|PubMed:28687655}; CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + 2 H2O = 3-beta-D-galactosyl-sn-glycerol + 2 a fatty acid + 2 H(+); Xref=Rhea:RHEA:13189, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15754, ChEBI:CHEBI:17615, ChEBI:CHEBI:28868; EC=3.1.1.26; Evidence={ECO:0000269|PubMed:28687655}; |
| DNA Binding | |
| EC Number | 3.1.1.32; 3.1.1.26 |
| Enzyme Function | FUNCTION: Sn-1-specific phospholipase A1 involved in seed oil biosynthesis. Hydrolyzes polyunsaturated acyl groups from a unique chloroplast-specific phosphatidylglycerol (PG) that contains 16:1 delta 3-trans as its second acyl group. The polyunsaturated acyl groups released by PLIP1 are exported from the chloroplast, reincorporated into phosphatidylcholine (PC), and ultimately enter seed triacylglycerol (TAG). In vitro, possesses broad substrate specificity. Can hydrolyze the galactolipid monogalactosyldiacylglycerol (MGDG), and the phoshpolipids phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA), phosphatidylserine (PS) phosphatidylglycerol (PG) and phosphatidylinositol (PI). {ECO:0000269|PubMed:28687655}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Erroneous gene model prediction (1); Motif (1); Mutagenesis (2); Transit peptide (1) |
| Keywords | Chloroplast;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Plastid;Reference proteome;Thylakoid;Transit peptide |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane {ECO:0000269|PubMed:28687655}; Peripheral membrane protein {ECO:0000305|PubMed:28687655}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 14576160; 17337630; 20348210; 23505340; 29666162; 31221730; |
| Motif | MOTIF 420..424; /note=GXSXG; /evidence=ECO:0000250|UniProtKB:Q948R1 |
| Gene Encoded By | |
| Mass | 71,735 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:18689; RHEA:13189 |
| Cross Reference Brenda |