| IED ID | IndEnz0005000088 |
| Enzyme Type ID | lipase000088 |
| Protein Name |
Sinapine esterase BnSCE3 EC 3.1.1.49 Lipase 2 BnLIP2 |
| Gene Name | |
| Organism | Brassica napus (Rape) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Brassiceae Brassica Brassica napus (Rape) |
| Enzyme Sequence | MASSLKKLITSFLLFFFYTIIVASSEPSCRRYKSIISFGDSIADTGNYLHLSDVNHPPQAAFLPYGETFFSVPTGRDSDGRLIIDFIAEFLGLPYVPPYFGSQNVSFEQGVNFAVYGATALDRAFFIEKGIVSDFTNVSLSVQLNTFKQILPTLCASSSRDCREMLGDSLILMGESGGNDYNYPFFEDKSINEIKELTPLIIKAISDAIVDLIDLGGKTFLVPGSFPVGCSAAYLTLFQTAKEKDYDPLTGCLPWLNDFGKHHDEQLKTEIRRLRKLYPHVNIMYADYYNSLYRLYQKPTKYGFKNRPLAACCGVGGQYNFTIGEECGYEGVGYCQNPSEYINWDGYHITEAAHQKMAHGILNGPYATPAFNWSCLDAASVDNESSFGS |
| Enzyme Length | 389 |
| Uniprot Accession Number | Q3ZFI4 |
| Absorption | |
| Active Site | ACT_SITE 41; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:Q00017; ACT_SITE 345; /evidence=ECO:0000250|UniProtKB:Q00017; ACT_SITE 348; /evidence=ECO:0000250|UniProtKB:Q00017 |
| Activity Regulation | ACTIVITY REGULATION: Inhibited by PMSF. {ECO:0000269|PubMed:18036206}. |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=H2O + O-sinapoylcholine = choline + E-sinapate + H(+); Xref=Rhea:RHEA:10016, ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16353, ChEBI:CHEBI:30023; EC=3.1.1.49; Evidence={ECO:0000269|PubMed:18036206}; |
| DNA Binding | |
| EC Number | 3.1.1.49 |
| Enzyme Function | FUNCTION: Sinapine esterase that catalyzes that hydrolysis of sinapine, releasing choline and sinapate. Sinapine (O-sinapoylcholine) is the predominant phenolic compound in a complex group of sinapate esters in seeds of oilseed rape (B.napus). Sinapine has antinutritive activity and prevents the use of seed protein for food and feed. Shows broad substrate specificity towards various other choline esters, including phosphatidylcholine. {ECO:0000269|PubMed:18036206, ECO:0000269|PubMed:21248075}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Glycosylation (5); Signal peptide (1) |
| Keywords | Glycoprotein;Hydrolase;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..25; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 43,319 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=2.6 uM for sinapine {ECO:0000269|PubMed:18036206}; KM=11 uM for cinnamoylcholine {ECO:0000269|PubMed:18036206}; KM=101 uM for 3-phenylpropionylcholine {ECO:0000269|PubMed:18036206}; |
| Metal Binding | |
| Rhea ID | RHEA:10016 |
| Cross Reference Brenda | 3.1.1.49; |