| IED ID | IndEnz0005000089 |
| Enzyme Type ID | lipase000089 |
| Protein Name |
Senescence-associated carboxylesterase 101 EC 3.1.1.1 |
| Gene Name | SAG101 At5g14930 F2G14.50 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MESSSSLKGSALGKLVVTSGLLHSSWSKILEIHNPPYSNHDPGLQVSKKKKDSGLEFQIHREEKFTLVVFSAPPICRSSSSDSTLLHVKDKENPFPFLCSENNPSFSLHTPAFNLFTSASTSLTYLKSELLQTLKSEKPVIITGAALGGSVASLYTLWLLETIEPTLKRPLCITFGSPLIGDASLQQILENSVRNSCFLHVVSAQTRIKMDFFKPFGTFLICFDSGCVCIEDHVAVTELLNGVHDSGLVDYSQVLNRLDQSMLSLADSRLIPEDVIKGIEKRAEMKNLRFDMMFKKLNDMKISMAYIEWYKKKCKEVKIGYYDRFKTQLAFPSKEFDINIKNHHKSELNRFWKSVVEEVERRPQSDASILKRRFLFSGNNYRRMIEPLDIAEYYLEGRKEYRTTGRSHHYVMLEKWFGMESILIEKERCKKRDLSDLLTFDSCFWAEVEDSLIVINQLNTTVGMRDDVREVLTRKLVEFEGYVWEIITKREVSPEIFLEESSFMKWWKEYKKIKGFNSSYLTEFMNTRKYESYGKSQ |
| Enzyme Length | 537 |
| Uniprot Accession Number | Q4F883 |
| Absorption | |
| Active Site | |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000269|PubMed:11971136}; |
| DNA Binding | |
| EC Number | 3.1.1.1 |
| Enzyme Function | FUNCTION: Acyl hydrolase that triggers the leaf senescence onset. Can use triolein as substrate to produce oleic acids.; FUNCTION: Involved in the EDS1-dependent intrinsic and indispensable resistance signaling pathway; together with PAD4, required for programmed cell death triggered by RPS4 in response to avirulent pathogens (e.g. P.syringae pv. tomato strain DC3000 and H.parasitica isolates CALA2 and EMWA1) and in restricting the growth of virulent pathogens (e.g. H.parasitica isolates NOCO2 and P.syringae pv. tomato strain DC3000 avrRps4). Contributes in reinforcing the immune response around hypersensitive response foci (PubMed:21434927). Regulates the nuclear localization of EDS1. Essential for the RPP8/HRT-mediated resistance to the turnip crinkle virus (TCV). Involved in the post-invasion resistance to P.pachyrhizi in the mesophyll. {ECO:0000269|PubMed:21434927, ECO:0000269|PubMed:22072959}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Alternative sequence (2); Beta strand (11); Chain (1); Erroneous gene model prediction (1); Helix (27); Mutagenesis (4); Sequence conflict (1); Signal peptide (1); Transmembrane (1); Turn (3) |
| Keywords | 3D-structure;Alternative splicing;Cytoplasm;Hydrolase;Lipid degradation;Lipid metabolism;Membrane;Nucleus;Plant defense;Reference proteome;Signal;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | INDUCTION: Specifically induced during senescence via a complex epigenetic processe involving histone methylation. {ECO:0000269|PubMed:19143996}. |
| Subcellular Location | SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}. Nucleus {ECO:0000269|PubMed:22072959}. Cytoplasm {ECO:0000269|PubMed:22072959}. Note=Can move to the cytoplasm when in complex with PAD4 and EDS1. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..27; /evidence=ECO:0000255 |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 4NFU; |
| Mapped Pubmed ID | 15282545; 15604721; 15990873; 16299169; 16514542; 16603661; 16913859; 17142483; 17227549; 17885809; 18650403; 18705666; 19578402; 23505340; 25681297; 25740259; 25915452; 26149542; 26340231; 26505534; 28351989; 28475615; 29253890; 29931201; 31311833; 31550533; 32396762; 34099661; |
| Motif | |
| Gene Encoded By | |
| Mass | 62,066 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21164 |
| Cross Reference Brenda |