| IED ID | IndEnz0005000098 |
| Enzyme Type ID | lipase000098 |
| Protein Name |
Patatin-like protein 3 AtPLP3 EC 3.1.1.- Patatin-related phospholipase A IIbeta pPLAIIb Phospholipase A IVC AtPLAIVC |
| Gene Name | PLP3 At4g37050 AP22.16 C7A10.310 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MDTERGSISSSEISRTAHLQDRTVACLPPSYGQLVTILSIDGGGIRGIIPGTILAYLESQLQELDGEEARLVDYFDVISGTSTGGLIVAMLTAQDQSGGHSRNSNRPLFEAKEIVPFYLKHSPKIFPQPRGIFCGWGETIVRLVGGPKFNGKYLHDLVEGFLGDTKLTQSLTNVVIPCFDIKKLQPVIFSSYQAVNNQAMNAKLSDICISTSAAPTFFPAHRFTNEDSEGIKHEFNLIDGGIAANNPTLCAIAEVTKQIIKKNPVMGDISPLDFTRFLVISIGTGSIRNQEKYNAKMASKWGLMCWVFESGSTPILDCYSEAIHDMVDYQSSVVFQALRSEKNYLRIDDDSLKGDLGSVDISTEKNMEGLVEVGEALLKKRVSRVNLESGHYQPISENVTNEEALKRFAKVLSEERKLRESRSPKLKI |
| Enzyme Length | 428 |
| Uniprot Accession Number | O23181 |
| Absorption | |
| Active Site | ACT_SITE 82; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; ACT_SITE 239; /note=Proton acceptor; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Possesses non-specific lipolytic acyl hydrolase (LAH) activity. Catalyzes the hydrolysis of the neutral lipids monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG) and phosphatidylglycerol (PG), and less efficiently the polar lipids phosphatidylcholine (PC) and phosphatidylinositol (PI), but not the storage lipid triacylglycerol (TAG). May play a role in root development. {ECO:0000269|PubMed:20053799}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (2); Chain (1); Domain (1); Modified residue (1); Motif (3) |
| Keywords | Cytoplasm;Hydrolase;Lipid degradation;Lipid metabolism;Phosphoprotein;Plant defense;Reference proteome |
| Interact With | |
| Induction | INDUCTION: By abscisic acid (ABA) or phosphate deficiency in roots. {ECO:0000269|PubMed:20053799}. |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12226489}. |
| Modified Residue | MOD_RES 423; /note=Phosphoserine; /evidence=ECO:0000250|UniProtKB:O23179 |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12805597; 15130548; 16504176; 17475618; 18772308; 21447788; 23505340; |
| Motif | MOTIF 42..47; /note=GXGXXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 80..84; /note=GXSXG; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161; MOTIF 239..241; /note=DGA/G; /evidence=ECO:0000255|PROSITE-ProRule:PRU01161 |
| Gene Encoded By | |
| Mass | 47,204 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda | 3.1.1.23; |