| IED ID | IndEnz0005000149 |
| Enzyme Type ID | lipase000149 |
| Protein Name |
Triacylglycerol lipase 2 EC 3.1.1.3 |
| Gene Name | LIP2 At5g14180 MUA22.18 MUA22.19 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MAGSVMVPSVSIGLALSVLIFFALSLKTLEARGTFGRLAGQPPQRTAAGGICASSVHIFGYKCEEHDVVTQDGYILNMQRIPEGRAGAVAGDGGKRQPVLIQHGILVDGMSWLLNPADQNLPLILADQGFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLPAMFDHIHGLTGQKIHYLGHSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIGDIAAKTFLAEATSILGWPEFNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNASTIDLFLANEPQSTSTKNMIHLAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDVEFLLDQFKYHDIDKMNVQFVKDYAHADFIMGVTAKDVVYNQVATFFKRQA |
| Enzyme Length | 418 |
| Uniprot Accession Number | Q67ZU1 |
| Absorption | |
| Active Site | ACT_SITE 190; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 360; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 393; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; |
| DNA Binding | |
| EC Number | 3.1.1.3 |
| Enzyme Function | FUNCTION: Triacylglycerol (TAG) lipase. May be involved for TAG storage breakdown during seed germination (By similarity). {ECO:0000250}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Erroneous gene model prediction (2); Erroneous initiation (1); Glycosylation (3); Sequence conflict (3); Signal peptide (1) |
| Keywords | Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome;Secreted;Signal |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000305}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..31; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 12535340; 14982972; 15319476; 16226259; 18775970; 18783601; 19291320; 21105927; 23505340; |
| Motif | |
| Gene Encoded By | |
| Mass | 46,048 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:12044 |
| Cross Reference Brenda |