IED ID | IndEnz0005000162 |
Enzyme Type ID | lipase000162 |
Protein Name |
Lipase 2 EC 3.1.1.3 Glycerol ester hydrolase 2 |
Gene Name | lip2 geh MW0297 |
Organism | Staphylococcus aureus (strain MW2) |
Taxonomic Lineage | cellular organisms Bacteria Terrabacteria group Firmicutes Bacilli Bacillales Staphylococcaceae Staphylococcus Staphylococcus aureus Staphylococcus aureus (strain MW2) |
Enzyme Sequence | MLRGQEERKYSIRKYSIGVVSVLAATMFVVSSHEAQASEKTPTNAAVQKETLNQPGEQGNAITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQLSKYQSTQNSKTIRTQNDNQVKQDSERQGSKQSHQNNATNNTERQNDQVQNTHHAERNGSQSTTSQSNDVDKSQPSIPAQKVLPNHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDTHQPAHQIIDAKQDDTVRQSEQKPQVGDLSKHIDGQNSPEKPTDKNTDNKQLIKDALQAPKTRSTTNAAADAKKVRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNKFKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTGGHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGFKQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVSSHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFVNVTNDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATESKGTQLKAS |
Enzyme Length | 690 |
Uniprot Accession Number | Q8NYC2 |
Absorption | |
Active Site | ACT_SITE 412; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 603; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 645; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037 |
Activity Regulation | |
Binding Site | |
Calcium Binding | |
catalytic Activity | CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3; |
DNA Binding | |
EC Number | 3.1.1.3 |
Enzyme Function | |
Temperature Dependency | |
PH Dependency | |
Pathway | |
nucleotide Binding | |
Features | Active site (3); Chain (1); Compositional bias (6); Metal binding (5); Propeptide (1); Region (1); Signal peptide (1) |
Keywords | Calcium;Hydrolase;Lipid degradation;Lipid metabolism;Metal-binding;Secreted;Signal;Zymogen |
Interact With | |
Induction | |
Subcellular Location | SUBCELLULAR LOCATION: Secreted {ECO:0000250}. |
Modified Residue | |
Post Translational Modification | |
Signal Peptide | SIGNAL 1..37; /evidence=ECO:0000255 |
Structure 3D | |
Cross Reference PDB | - |
Mapped Pubmed ID | - |
Motif | |
Gene Encoded By | |
Mass | 76,522 |
Kinetics | |
Metal Binding | METAL 579; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 644; /note=Calcium; /evidence=ECO:0000250; METAL 647; /note=Calcium; /evidence=ECO:0000250; METAL 652; /note=Calcium; /evidence=ECO:0000250; METAL 655; /note=Calcium; /evidence=ECO:0000250 |
Rhea ID | RHEA:12044 |
Cross Reference Brenda |