Detail Information for IndEnz0005000175
IED ID IndEnz0005000175
Enzyme Type ID lipase000175
Protein Name Lipase 5
EC 3.1.1.3
Gene Name LIP5
Organism Diutina rugosa (Yeast) (Candida rugosa)
Taxonomic Lineage cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetales incertae sedis Diutina Diutina rugosa (Yeast) (Candida rugosa)
Enzyme Sequence MKLALALSLIASVAAAPTATLANGDTITGLNAIINEAFLGIPFAEPPVGNLRFKDPVPYRGSLNGQSFTAYGPSCMQQNPEGTYEENLPKVALDLVMQSKVFQAVLPNSEDCLTINVVRPPGTKAGANLPVMLWIFGGGFEIGSPTIFPPAQMVSKSVLMGKPIIHVAVNYRLASFGFLAGPDIKAEGSSNAGLKDQRLGMQWVADNIAGFGGDPSKVTIFGESAGSMSVLCHLLWNGGDNTYKGKPLFRAGIMQSGAMVPSDPVDGTYGTQIYDTLVASTGCSSASNKLACLRGLSTQALLDATNDTPGFLSYTSLRLSYLPRPDGANITDDMYKLVRDGKYASVPVIIGDQNDEGFLFGLSSLNTTTEADAEAYLRKSFIHATDADITALKAAYPSDVTQGSPFDTGILNALTPQLKRINAVLGDLTFTLSRRYFLNHYTGGPKYSFLSKQLSGLPILGTFHANDIVWQHFLLGSGSVIYNNAFIAFATDLDPNTAGLSVQWPKSTSSSQAGDNLMQISALGLYTGKDNFRTAGYNALFADPSHFFV
Enzyme Length 549
Uniprot Accession Number P32949
Absorption
Active Site ACT_SITE 224; /note=Acyl-ester intermediate; /evidence=ECO:0000255|PROSITE-ProRule:PRU10039; ACT_SITE 356; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 464; /note=Charge relay system; /evidence=ECO:0000250
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
DNA Binding
EC Number 3.1.1.3
Enzyme Function
Temperature Dependency
PH Dependency
Pathway
nucleotide Binding
Features Active site (3); Chain (1); Disulfide bond (2); Glycosylation (2); Signal peptide (1)
Keywords Disulfide bond;Glycoprotein;Hydrolase;Lipid degradation;Lipid metabolism;Signal
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..15
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 58,420
Kinetics
Metal Binding
Rhea ID RHEA:12044
Cross Reference Brenda