Detail Information for IndEnz0005000181
IED ID IndEnz0005000181
Enzyme Type ID lipase000181
Protein Name Lipase
EC 3.1.1.3
Triacylglycerol lipase
Gene Name
Organism Pseudomonas fluorescens
Taxonomic Lineage cellular organisms Bacteria Proteobacteria Gammaproteobacteria Pseudomonadales Pseudomonadaceae Pseudomonas Pseudomonas fluorescens group (fluorescent pseudomonads) Pseudomonas fluorescens
Enzyme Sequence MGVFDYKNLGTEASKTLFADATAITLYTYHNLDNGFAVGYQQHGLGLGCRHTGRGVARQHRLPGSDPPAFPGILTRKRPPWTRCTQPVGRQSSASALGYGGKVDARGTFFGEKAGYTTAQAEVLGKYDDAGKLLEIGIGFRGTSGPRESLITTPCRSGQRPARRAGPQGLCEKLCRRTFGGLLKTVADYAGAHGLSGKDVLVSGHSLGGLAVNSMADLSTSKWAGFYKDANYLAYASPTQSAGDKVLNIGYENDPVFRALDGSTFNLSSLGVHDKAHESTTDNIVSFNDHYASTLWNVLPFSIANLSTWVSHLPSAYGDGMTRVLESGFYEQMTRDSTIILCPTWSDPARANTWVQDLNRNAEPHTGNTFIIGSDGNDLIQGGKGADFIEGGKGNDTIRDNSGHNTFLFSGHFGQDRIIGYQPTGWCSRAPTAAPTCATTRRPWGPIRC
Enzyme Length 449
Uniprot Accession Number P26504
Absorption
Active Site ACT_SITE 206; /note=Charge relay system; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037
Activity Regulation
Binding Site
Calcium Binding
catalytic Activity CATALYTIC ACTIVITY: Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
DNA Binding
EC Number 3.1.1.3
Enzyme Function
Temperature Dependency BIOPHYSICOCHEMICAL PROPERTIES: Temperature dependence: Thermostable.;
PH Dependency
Pathway
nucleotide Binding
Features Active site (1); Chain (1); Metal binding (3); Region (1); Repeat (2); Signal peptide (1)
Keywords Calcium;Direct protein sequencing;Hydrolase;Lipid degradation;Lipid metabolism;Metal-binding;Repeat;Signal
Interact With
Induction
Subcellular Location
Modified Residue
Post Translational Modification
Signal Peptide SIGNAL 1..23; /evidence=ECO:0000255
Structure 3D
Cross Reference PDB -
Mapped Pubmed ID -
Motif
Gene Encoded By
Mass 48,233
Kinetics
Metal Binding METAL 318; /note=Calcium; via carbonyl oxygen; /evidence=ECO:0000250; METAL 387; /note=Calcium; /evidence=ECO:0000250; METAL 396; /note=Calcium; /evidence=ECO:0000250
Rhea ID RHEA:12044
Cross Reference Brenda