| IED ID | IndEnz0005000194 |
| Enzyme Type ID | lipase000194 |
| Protein Name |
Isoamyl acetate-hydrolyzing esterase EC 3.1.1.112 |
| Gene Name | iah1 SPCC126.10 |
| Organism | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota Taphrinomycotina Schizosaccharomycetes Schizosaccharomycetales Schizosaccharomycetaceae Schizosaccharomyces Schizosaccharomyces pombe (Fission yeast) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
| Enzyme Sequence | MSDSQDKNYISFEKTEIGQIVSEAPAEKYVSFQKKYFNRLTIIGDSITQKGFTPGGYCAELMNFYQRRLRVDVWGFSGYTSRHVLRYLPEIPLEIDSTKLLIVFLGTNDCQLTETGYMCPVDEFKNNLLALTRPFPHSKIIIVSPGICTKDICFKREQEPYVIAASETVNTLNKSKANSAGLINLYEITKSYPTPELLFTDGLHFSSLGYSLLFNEIVATISKAWPELLPNNLPLQFPHWSEILFT |
| Enzyme Length | 246 |
| Uniprot Accession Number | O74648 |
| Absorption | |
| Active Site | ACT_SITE 46; /note=Nucleophile; /evidence=ECO:0000250|UniProtKB:P41734; ACT_SITE 201; /note=Proton donor; /evidence=ECO:0000250|UniProtKB:P41734; ACT_SITE 204; /note=Proton acceptor; /evidence=ECO:0000250|UniProtKB:P41734 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=3-methylbutyl acetate + H2O = 3-methylbutanol + acetate + H(+); Xref=Rhea:RHEA:60436, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15837, ChEBI:CHEBI:30089, ChEBI:CHEBI:31725; EC=3.1.1.112; Evidence={ECO:0000250|UniProtKB:P41734}; |
| DNA Binding | |
| EC Number | 3.1.1.112 |
| Enzyme Function | |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Site (2) |
| Keywords | Cytoplasm;Hydrolase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 18684775; 19547744; 20473289; 23697806; 25452419; |
| Motif | |
| Gene Encoded By | |
| Mass | 27,991 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:60436 |
| Cross Reference Brenda |