| IED ID | IndEnz0005000259 |
| Enzyme Type ID | lipase000259 |
| Protein Name |
Phospholipase A1-IIgamma EC 3.1.1.- DAD1-like seedling establishment-related lipase AtDSEL Phospholipase DSEL |
| Gene Name | DSEL At4g18550 F28J12.210 |
| Organism | Arabidopsis thaliana (Mouse-ear cress) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids malvids Brassicales Brassicaceae Camelineae Arabidopsis Arabidopsis thaliana (Mouse-ear cress) |
| Enzyme Sequence | MKRKKKEEEEEKLIVTREFAKRWRDLSGQNHWKGMLQPLDQDLREYIIHYGEMAQAGYDTFNINTESQFAGASIYSRKDFFAKVGLEIAHPYTKYKVTKFIYATSDIHVPESFLLFPISREGWSKESNWMGYVAVTDDQGTALLGRRDIVVSWRGSVQPLEWVEDFEFGLVNAIKIFGERNDQVQIHQGWYSIYMSQDERSPFTKTNARDQVLREVGRLLEKYKDEEVSITICGHSLGAALATLSATDIVANGYNRPKSRPDKSCPVTAFVFASPRVGDSDFRKLFSGLEDIRVLRTRNLPDVIPIYPPIGYSEVGDEFPIDTRKSPYMKSPGNLATFHCLEGYLHGVAGTQGTNKADLFRLDVERAIGLVNKSVDGLKDECMVPGKWRVLKNKGMAQQDDGSWELVDHEIDDNEDLDF |
| Enzyme Length | 419 |
| Uniprot Accession Number | O49523 |
| Absorption | |
| Active Site | ACT_SITE 236; /note=Acyl-ester intermediate; /evidence=ECO:0000250; ACT_SITE 236; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 302; /note=Charge relay system; /evidence=ECO:0000250; ACT_SITE 339; /note=Charge relay system; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Acylhydrolase that catalyzes the hydrolysis of 1,3-diacylglycerol (1,3-DAG) and 1-monoacylglycerol (1-MAG) at the sn-1 position. High activity toward 1,3-DAG and 1-MAG, but low activity toward 1,2-diacylglycerol (1,2-DAG) and 1-lysophosphatidylcholine (1-LPC), and no activity toward phosphatidylcholine (PC), monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), triacylglycerol (TAG) and 2-monoacylglycerol (2-MAG). May be involved in the negative regulation of seedling establishment by inhibiting the breakdown, beta-oxidation and mobilization of seed storage oils. {ECO:0000269|PubMed:21477884}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 5-6 with 1,3-DAG as substrate and at 30 degrees Celsius. {ECO:0000269|PubMed:21477884}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (4); Beta strand (12); Chain (1); Coiled coil (2); Erroneous initiation (1); Helix (17); Turn (7) |
| Keywords | 3D-structure;Coiled coil;Cytoplasm;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:21477884}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 2YIJ; |
| Mapped Pubmed ID | 18230180; 23505340; |
| Motif | |
| Gene Encoded By | |
| Mass | 47,792 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |