| IED ID | IndEnz0005000270 |
| Enzyme Type ID | lipase000270 |
| Protein Name |
Lipid droplet-associated hydrolase EC 3.1.1.- Lipid droplet-associated serine hydrolase |
| Gene Name | sturkopf CG9186 |
| Organism | Drosophila melanogaster (Fruit fly) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Metazoa Eumetazoa Bilateria Protostomia Ecdysozoa Panarthropoda Arthropoda Mandibulata Pancrustacea Hexapoda Insecta Dicondylia Pterygota (winged insects) Neoptera Endopterygota Diptera Brachycera Muscomorpha Eremoneura Cyclorrhapha Schizophora Acalyptratae Ephydroidea Drosophilidae (pomace flies) Drosophilinae Drosophilini Drosophila (fruit flies) Sophophora melanogaster group melanogaster subgroup Drosophila melanogaster (Fruit fly) |
| Enzyme Sequence | MQEAYVNINSIPTHIFTWGRWIEETITEKEIVICITGNPGLPGFYTEFAGTLQKELGDLPVWVIGHAGHDDPPEASIREVPQLSGNEELFNLDGQIRHKIAFIEKYVPSDVKIHLIGHSIGAWMILQLLENERIRSRIQKCYMLFPTVERMMESPNGWVFTKVAMPLYSVFGYIFFSFFNFLPVWLRLMLIQIYFLIFSIPRQFLGTALKYSKPSVAEKVVFLADDEMARVRGIQREIVEQNLDLLKFYYGTTDGWVPISYYDQLKKDYPKVDAQLDTKKIDHAFVLRHSQPMAVIVRDMIQQHRRV |
| Enzyme Length | 307 |
| Uniprot Accession Number | Q9W0H3 |
| Absorption | |
| Active Site | ACT_SITE 119; /note=Nucleophile; /evidence=ECO:0000255|PROSITE-ProRule:PRU10037; ACT_SITE 254; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q9H6V9; ACT_SITE 283; /note=Charge relay system; /evidence=ECO:0000250|UniProtKB:Q9H6V9 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | |
| DNA Binding | |
| EC Number | 3.1.1.- |
| Enzyme Function | FUNCTION: Probable serine lipid hydrolase associated with lipid droplets (By similarity). Appears to lack cholesterol esterase activity (PubMed:27836991). Appears to lack triglyceride lipase activity (PubMed:23525007, PubMed:27836991). Involved in negatively regulating juvenile hormone (JH) and possibly, insulin signaling activities such as triacylglycerols (TAG) storage, and thereby plays a role in the endocrine regulation of organismal growth and survival (PubMed:30917324). Likely functions by enhancing the activity of the JH hydrolase enzymes Jheh1 and Jheh2 (PubMed:30917324). Required for lipid droplet positioning and fat storage (PubMed:23525007). {ECO:0000250|UniProtKB:Q8BVA5, ECO:0000269|PubMed:23525007, ECO:0000269|PubMed:27836991, ECO:0000269|PubMed:30917324}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Region (1); Transmembrane (2) |
| Keywords | Endoplasmic reticulum;Hydrolase;Lipid droplet;Membrane;Reference proteome;Transmembrane;Transmembrane helix |
| Interact With | |
| Induction | |
| Subcellular Location | SUBCELLULAR LOCATION: Lipid droplet {ECO:0000269|PubMed:23525007, ECO:0000269|PubMed:27836991}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:23525007}; Multi-pass membrane protein {ECO:0000255}. Note=Localizes to the endoplasmic reticulum in absence of lipid droplets and translocates to lipid droplets upon lipid storage induction. {ECO:0000269|PubMed:23525007}. |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | 16783380; 16979555; 18245369; 18419806; 18783608; 19317464; 20813047; 23071443; 23319140; 23765091; 25312911; 26212136; 27197209; 27894253; 27956707; 28742022; 31722958; 32335968; 32730754; 32903680; 33827210; 33945525; |
| Motif | |
| Gene Encoded By | |
| Mass | 35,736 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | |
| Cross Reference Brenda |