| IED ID | IndEnz0005000340 |
| Enzyme Type ID | lipase000340 |
| Protein Name |
2-hydroxyisoflavanone dehydratase EC 3.1.1.1 EC 4.2.1.105 Carboxylesterase HIDH |
| Gene Name | HIDH Glyma01g45020 |
| Organism | Glycine max (Soybean) (Glycine hispida) |
| Taxonomic Lineage | cellular organisms Eukaryota Viridiplantae Streptophyta Streptophytina Embryophyta Tracheophyta Euphyllophyta Spermatophyta Magnoliopsida Mesangiospermae eudicotyledons Gunneridae Pentapetalae rosids fabids Fabales Fabaceae Papilionoideae 50 kb inversion clade NPAAA clade indigoferoid/millettioid clade Phaseoleae Glycine Glycine subgen. Soja Glycine max (Soybean) (Glycine hispida) |
| Enzyme Sequence | MAKEIVKELLPLIRVYKDGSVERLLSSENVAASPEDPQTGVSSKDIVIADNPYVSARIFLPKSHHTNNKLPIFLYFHGGAFCVESAFSFFVHRYLNILASEANIIAISVDFRLLPHHPIPAAYEDGWTTLKWIASHANNTNTTNPEPWLLNHADFTKVYVGGETSGANIAHNLLLRAGNESLPGDLKILGGLLCCPFFWGSKPIGSEAVEGHEQSLAMKVWNFACPDAPGGIDNPWINPCVPGAPSLATLACSKLLVTITGKDEFRDRDILYHHTVEQSGWQGELQLFDAGDEEHAFQLFKPETHLAKAMIKRLASFLV |
| Enzyme Length | 319 |
| Uniprot Accession Number | Q5NUF3 |
| Absorption | |
| Active Site | ACT_SITE 164; /evidence=ECO:0000305|PubMed:15734910; ACT_SITE 263; /evidence=ECO:0000305|PubMed:15734910; ACT_SITE 295; /evidence=ECO:0000305|PubMed:15734910 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=(2R,3S)-2,4',7-trihydroxyisoflavanone = daidzein + H(+) + H2O; Xref=Rhea:RHEA:16445, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:63325, ChEBI:CHEBI:77764; EC=4.2.1.105; Evidence={ECO:0000269|PubMed:15734910}; CATALYTIC ACTIVITY: Reaction=2-hydroxy-2,3-dihydrogenistein = genistein + H(+) + H2O; Xref=Rhea:RHEA:36803, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:31080, ChEBI:CHEBI:74224; EC=4.2.1.105; Evidence={ECO:0000269|PubMed:15734910}; CATALYTIC ACTIVITY: Reaction=a carboxylic ester + H2O = a carboxylate + an alcohol + H(+); Xref=Rhea:RHEA:21164, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29067, ChEBI:CHEBI:30879, ChEBI:CHEBI:33308; EC=3.1.1.1; Evidence={ECO:0000269|PubMed:15734910}; |
| DNA Binding | |
| EC Number | 3.1.1.1; 4.2.1.105 |
| Enzyme Function | FUNCTION: Dehydratase that mediates the biosynthesis of isoflavonoids. Can use both 4'-hydroxylated and 4'-methoxylated 2-hydroxyisoflavanones as substrates. Has also a slight carboxylesterase activity toward p-nitrophenyl butyrate. {ECO:0000269|PubMed:15734910}. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis. |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Motif (1); Mutagenesis (5) |
| Keywords | Flavonoid biosynthesis;Hydrolase;Lyase;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | MOTIF 77..79; /note=Involved in the stabilization of the negatively charged intermediate by the formation of the oxyanion hole; /evidence=ECO:0000305|PubMed:15734910 |
| Gene Encoded By | |
| Mass | 35,138 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=29 uM for 2,7-dihydroxy-4'-methoxyisoflavanone (at pH 7.5 and 30 degrees Celsius) {ECO:0000269|PubMed:15734910}; KM=114 uM for 2,7,4'-trihydroxyisoflavanone (at pH 7.5 and 30 degrees Celsius) {ECO:0000269|PubMed:15734910}; KM=170 uM for 2,5,7,4'-tetrahydroxyisoflavanone (at pH 7.5 and 30 degrees Celsius) {ECO:0000269|PubMed:15734910}; Note=kcat is 1.6 sec(-1) with 2,7-dihydroxy-4'-methoxyisoflavanone, 5.3 sec(-1) with 2,7,4'-trihydroxyisoflavanone and 18.1 sec(-1) with 2,5,7,4'-tetrahydroxyisoflavanone as substrates, respectively (at pH 7.5 and 30 degrees Celsius).; |
| Metal Binding | |
| Rhea ID | RHEA:16445; RHEA:36803; RHEA:21164 |
| Cross Reference Brenda | 4.2.1.105; |