| IED ID | IndEnz0005000363 |
| Enzyme Type ID | lipase000363 |
| Protein Name |
Phosphatidylcholine-sterol acyltransferase EC 2.3.1.43 Glycerophospholipid-cholesterol acyltransferase GCAT |
| Gene Name | |
| Organism | Aeromonas hydrophila |
| Taxonomic Lineage | cellular organisms Bacteria Proteobacteria Gammaproteobacteria Aeromonadales Aeromonadaceae Aeromonas Aeromonas hydrophila |
| Enzyme Sequence | MKKWFVCLLGLVALTVQAADSRPAFSRIVMFGDSLSDTGKMYSKMRGYLPSSPPYYEGRFSNGPVWLEQLTNEFPGLTIANEAEGGPTAVAYNKISWNPKYQVINNLDYEVTQFLQKDSFKPDDLVILWVGANDYLAYGWNTEQDAKRVRDAISDAANRMVLNGAKEILLFNLPDLGQNPSARSQKVVEAASHVSAYHNQLLLNLARQLAPTGMVKLFEIDKQFAEMLRDPQNFGLSDTENACYGGSYVWKPFASRSASTDSQLSAFNPQERLAIAGNPLLAQAVASPMAARSASTLNCEGKMFWDQVHPTTVVHAALSEPAATFIESQYEFLAH |
| Enzyme Length | 335 |
| Uniprot Accession Number | P10480 |
| Absorption | |
| Active Site | ACT_SITE 34; /note=Nucleophile; /evidence=ECO:0000250; ACT_SITE 306; /evidence=ECO:0000250; ACT_SITE 309; /evidence=ECO:0000250 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + a sterol = a 1-acyl-sn-glycero-3-phosphocholine + a sterol ester; Xref=Rhea:RHEA:21204, ChEBI:CHEBI:15889, ChEBI:CHEBI:35915, ChEBI:CHEBI:57643, ChEBI:CHEBI:58168; EC=2.3.1.43; |
| DNA Binding | |
| EC Number | 2.3.1.43 |
| Enzyme Function | FUNCTION: Fatty acid transfer between phosphatidylcholine and cholesterol. |
| Temperature Dependency | |
| PH Dependency | |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Chain (1); Frameshift (1); Mutagenesis (2); Signal peptide (1) |
| Keywords | Acyltransferase;Cholesterol metabolism;Lipid metabolism;Signal;Steroid metabolism;Sterol metabolism;Transferase |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | SIGNAL 1..18; /evidence=ECO:0000255 |
| Structure 3D | |
| Cross Reference PDB | - |
| Mapped Pubmed ID | - |
| Motif | |
| Gene Encoded By | |
| Mass | 37,067 |
| Kinetics | |
| Metal Binding | |
| Rhea ID | RHEA:21204 |
| Cross Reference Brenda |