| IED ID | IndEnz0005000364 |
| Enzyme Type ID | lipase000364 |
| Protein Name |
Isoamyl acetate-hydrolyzing esterase EC 3.1.1.112 |
| Gene Name | IAH1 EST2 YOR126C O3287 YOR3287C |
| Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Taxonomic Lineage | cellular organisms Eukaryota Opisthokonta Fungi Dikarya Ascomycota saccharomyceta Saccharomycotina (true yeasts) Saccharomycetes Saccharomycetales Saccharomycetaceae Saccharomyces Saccharomyces cerevisiae (Baker's yeast) Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Enzyme Sequence | MDYEKFLLFGDSITEFAFNTRPIEDGKDQYALGAALVNEYTRKMDILQRGFKGYTSRWALKILPEILKHESNIVMATIFLGANDACSAGPQSVPLPEFIDNIRQMVSLMKSYHIRPIIIGPGLVDREKWEKEKSEEIALGYFRTNENFAIYSDALAKLANEEKVPFVALNKAFQQEGGDAWQQLLTDGLHFSGKGYKIFHDELLKVIETFYPQYHPKNMQYKLKDWRDVLDDGSNIMS |
| Enzyme Length | 238 |
| Uniprot Accession Number | P41734 |
| Absorption | |
| Active Site | ACT_SITE 12; /note=Nucleophile; /evidence=ECO:0000305|PubMed:21069734; ACT_SITE 187; /note=Proton donor; /evidence=ECO:0000305|PubMed:21069734; ACT_SITE 190; /note=Proton acceptor; /evidence=ECO:0000305|PubMed:21069734 |
| Activity Regulation | |
| Binding Site | |
| Calcium Binding | |
| catalytic Activity | CATALYTIC ACTIVITY: Reaction=3-methylbutyl acetate + H2O = 3-methylbutanol + acetate + H(+); Xref=Rhea:RHEA:60436, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15837, ChEBI:CHEBI:30089, ChEBI:CHEBI:31725; EC=3.1.1.112; Evidence={ECO:0000269|PubMed:10855721, ECO:0000269|PubMed:21069734, ECO:0000269|Ref.2}; |
| DNA Binding | |
| EC Number | 3.1.1.112 |
| Enzyme Function | FUNCTION: Plays a crucial role in the hydrolysis of isoamyl acetate in sake mash (Ref.2). Hydrolyzes short chain esters from acetate (C2) to hexanoate (C6), showing more specificity for shorter chain exters. No activity for decanoate (C10) esters (PubMed:21069734). {ECO:0000269|PubMed:21069734, ECO:0000269|Ref.2}. |
| Temperature Dependency | |
| PH Dependency | BIOPHYSICOCHEMICAL PROPERTIES: pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:10855721}; |
| Pathway | |
| nucleotide Binding | |
| Features | Active site (3); Beta strand (6); Chain (1); Helix (13); Mutagenesis (1); Site (2); Turn (4) |
| Keywords | 3D-structure;Hydrolase;Lipid degradation;Lipid metabolism;Reference proteome |
| Interact With | |
| Induction | |
| Subcellular Location | |
| Modified Residue | |
| Post Translational Modification | |
| Signal Peptide | |
| Structure 3D | X-ray crystallography (1) |
| Cross Reference PDB | 3MIL; |
| Mapped Pubmed ID | 16002012; 22940803; 24384752; |
| Motif | |
| Gene Encoded By | |
| Mass | 27,346 |
| Kinetics | BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=40.3 mM for 3-mehtylbutyl acetate {ECO:0000269|PubMed:10855721}; |
| Metal Binding | |
| Rhea ID | RHEA:60436 |
| Cross Reference Brenda | 3.1.1.112; |